Background:The increasing incidence of papillary thyroid carcinoma (PTC) and the inadequacy of routine histologic examination in its diagnosis necessitate the application of ancillary studies like immunohistochemistry. This research aimed to investigate the scoring system and diagnosis of PTC with cytokeratin 19 (CK19), human bone marrow endothelium marker-1 (HBME-1), and galectin-3.Methods: This experimental laboratory study was performed at Babol University of Medical Sciences, Mazandaran, Iran from April 2017 to March 2019. Neoplastic and nonneoplastic tissue samples of 100 cases with a diagnosis of PTC were selected by convenience sampling. CK19, HBME-1, and galectin-3 immunohistochemistry markers were used on tissue samples. Analysis was performed using the t test and the chi-square test, as well as the receiver operator characteristic (ROC) curve (significance level P < 0.05).Results: The CK19 staining was observed in all 100 (100%) non-neoplastic tissues, but HBME-1 and galectin-3 were positive in 36 (36%) and 14 (14%) of non-neoplastic tissues, respectively. The intensity scores of all the markers and their total had significantly different means in PTC and non-neoplastic tissues (P < 0.001). A significant difference was observed between the total score of each marker and the total score of their combination (P < 0.001). The combination of all 3 markers with an 11.5 0 cut-off for the total score showed the most sensitive (0.99) and specific (1.00) results.Conclusion: Interpreting CK19, HBME-1, and galectin-3 with the aid of the proposed scoring system was fruitful. HBME-1 and galectin-3 can be used individually or in combination for the diagnosis of PTC.

Introduction: Idiopathic nephrotic syndrome has been considered a cell-mediated immunity disorder with T cell dysfunction. Objectives: The purpose of this study was to investigate immunologic dysfunction in children with steroid-sensitive nephrotic syndrome (SSNS) by evaluating serum interleukin-18 (IL-18). Patients and Methods: A total of 30 children with SSNS (n=20 males, n=10 females), aged 1–11 years were enrolled in this study. All of them were treated with steroids at the time of study. Serum IL-18 was compared during both active and remission phases of nephrotic syndrome. In addition, correlation between serum IL-18 with urine protein and serum biochemical variables was evaluated. Results: Mean serum IL-18 was higher in the active phase, and decreased significantly poststeroid treatment (4.87±1.43 ng/L versus 2.39± 0.94 ng/L; P=0.01). It had no correlation with other variables. Conclusion: According to increased serum IL-18 level, dysregulation of type-1 and type-2 mediated immune reactions is suggested in children with SSNS.