It is still unclear whether different HHV-8 genotypes may have different pathogenic and tumorigenic properties associated with a diverse rate of disease progression. In some areas where genotype C was found to be prominent among classic KS patients, genotype A was shown to be more frequent among AIDS-associated KS patients. Genotype C was previously reported to be widespread in Iran, with genotype A being less frequent among patients with classic KS although no data are available with regards to the HHV-8 genotyping among Iranian HIV-infected patients. In order to analyze HHV-8 genotypes (ORF K1), six HIV-infected patients (with or without KS), and 22 HIV-negative subjects (classic/iatrogenic KS patients and IVDUs) were investigated using nested PCR. Genotype A was detected more frequently among HIV-infected patients with or without KS (three out of six) whereas genotype C was found more common among HIV-negative subjects including classic/iatrogenic KS patients and IVDSs (21 out of 22), and this difference was statistically significant (P = 0.044). In conclusion, our data further support the dominancy of HHV-8 genotype C in Iranian general population. Moreover, genotype A was more common among HIV-infected patients with or without KS. J. Med. Virol. 89:703-709, 2017. © 2016 Wiley Periodicals, Inc.

Interleukin-8 (IL8) is the protein released by different cells. Increased of IL8 indicated the infection in any part of body so increased of IL8 in urine is indicated the urinary tract infection. Patients with spinal cord injuries have to use catheter for long time and or for all of life, and they are high risk to recurrent urinary tract infections and other infections. To determine of increasing urinary IL8 in patients with spinal cord injuries the present study is the survey of this. Clean catch midstream urine samples were collected from spinal cord injury patients who admitted to "Brain and Spinal Injury Repair Research Centre" in Imam Hospital in duration almost one year. Urine samples were collected from 97 spinal cord injury patients included 85 male and 12 female and then were analyzed by biochemical and microscopically surveys and then, IL8 were measured in samples by ELISA method. In total 97 patients, 87 patients were IL8 positive (>10 pg/ml) and in 87 positive samples, 59 patients had bacteriuria and 54 patients had pyuria. Statistical analysis showed that the significant relationship between bacteriuria and pyuria with urinary IL8 positive patients (P<0.05). The patients with spinal cord injury need to monitor for urinary tract infection although without any symptoms of infections and sometimes. The measurement of IL8 is a fast and easy method for monitoring of urinary tract infection in these patients.

A single-nucleotide polymorphism (SNP) in the promoter region of MDM2 (SNP309T>G, rs2279744) has been shown to increase the expression of the MDM2 protein in various cancer types. However, only one study has analyzed the role of the MDM2 polymorphism in the development of Kaposi's sarcoma (KS). The association of MDM2 SNP309 with classic KS risk was evaluated in 79 Iranian patients with classic KS and 123 healthy controls. The MDM2 SNP309 was genotyped using PCR and restriction fragment length polymorphism methods. No significant correlation was found between the SNP309 polymorphism in MDM2 promoter and classic KS risk. There was no significant correlation between gender and disease stage. However, a significant association was found between SNP309 GG genotype and younger age (≤50 years) (odds ratio 9.5, 95% confidence intervals 1.5-60, p = 0.03). Our findings support no major role for the MDM2 SNP309 in KS development although it might influence the clinical outcome of KS in younger patients.

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