The conventional clinical diagnostic aids which were introduced almost half a century ago continues to function as the basis of diagnosis in clinical practice. Recent advances in biochemistry and molecular sciences brought us to the significance of biomarkers as diagnostic aids. This new advance in medical science for periodontal diagnosis will provide immense benefit in managing periodontitis patients. Biomarkers are tell -tale molecules that can be used to monitor health status, disease onset, treatment response and outcome. These biomarkers can be obtained from various body fluids. Obtaining GCF by non invasive method provides an easy and reliable modality in using GCF as potential source for biomarkers This simple and non-invasive nature of GCF sourcing and its high sensitivity assay development have led to the idea of using the GCF biomarkers as diagnostic aids for periodontal diagnosis.