Ethanol extracts of 19 Malaysian traditional vegetables (locally known as 'ulam')

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1. Zearalenone, a fungal oestrogenic compound, was subjected to microbial transformation studies. Preliminary screening with 150 fungal species showed that Gliocladium roseum was capable of metabolizing zearalenone in 80-90% yields. 2. Large-scale fermentation with G. roseum produced a 1:1 mixture of 1-(3,5-dihydroxyphenyl)-10'-hydroxy-1-undecen-6'-one and 1-(3,5-dihydroxyphenyl)-6'-hydroxy-1-undecen-10'-one. The compounds were isolated and purified at -20 degrees C, and identified using spectroscopic analysis and by comparison to products obtained from alkaline hydrolysis of zearalenone.

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