Antimicrobial and Cytotoxic Properties of Some Malaysian Traditional Vegetables (Ulam)

Mackeen, Muhammad Mukram Mohamed; Ali, Abdul Manaf; El-Sharkawy, Saleh; Manap, Mohd Yazid Abdul; Salleh, Khalijah Mohd; Lajis, Nordin Hj; Kawazu, Kazuyoshi

doi:10.1076/phbi.35.3.174.13294

Cited by 120 publications

(81 citation statements)

References 6 publications

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“…Ethanol extract of the leaves of E. elatior showed activity against bacteria gram-positive (Bacillus cereus and Bacillus megatrium) and bacteria gram negative (Escherichia coli and Pseudomonas aeruginosa). The results revealed that E. elatior showed antibacterial activity with minimum concentration 100-800 mg/mL and the minimum lethal concentration 400-800 mg/mL [6]. In addition, E. elatior had antibacterial activity against S. aureus with minimum inhibitory concentration values about 15% of concentrations at with the percentage of inhibition about 95.63%, E. coli by 50% with the percent inhibition of 92.41%.…”

Section: Introductionmentioning

confidence: 72%

Characterization of Chemical Constituent and Antibacterial Activity of Honje Fruit Skin (Etlingera elatior)

Sukandar¹,

Fitriyanti²,

Amelia³

et al. 2018

Proceedings of the International Conference on Science and Technology (ICOSAT 2017)

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Characteristic of chemical constituent and antibacterial property from Etlingera elatior fruit have been carried out. Essential oil of plant was extracted using steam and water distillation and then analyzed by gas chromatography-mass spectrometry. Antibacterial activity of the essential oil was done through disc diffusion method against Bacillus cereus, Staphylococcus aureus, Eschericia coli and Pseudomonas aeruginosa. Percentage yield of essential oil was about 0,09% (v/w). The GC-MS profile revealed that E. elatior contained 35 compounds where 1-dodecanol, dodecanal and trans-caryophyllene were predicted to be main components. The oil showed activity against B. Cereus, S. Aureus, E. Coli, and P. aeruginosa with zone of inhibition were about 25, 15.2, 11.67 and 12.33 mm respectively.

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Section: Introductionmentioning

confidence: 72%

Characterization of Chemical Constituent and Antibacterial Activity of Honje Fruit Skin (Etlingera elatior)

Sukandar¹,

Fitriyanti²,

Amelia³

et al. 2018

Proceedings of the International Conference on Science and Technology (ICOSAT 2017)

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“…Antibacterial assay using a disc diffusion method Antibacterial activity was measured using a disc diffusion method described by Mackeen et al [21]. Standard antibiotic, streptomycin was used as positive control.…”

Section: Qualitative Analysis Of Secondary Metabolitesmentioning

confidence: 99%

Antibacterial, antioxidant and anticancer properties of Turbinaria conoides (J. Agardh) Kuetz

et al. 2017

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Background: Cancer causes leading death in the world population due to exposure of various carcinogenic/mutagenic agents, radiation and life style. There are 2.6 million new cases diagnosed each year. Therefore, the objective is aimed to study the antibacterial and antioxidant activities of solvent fractions of ethanolic extract of T. conoides and its anticancer activity also evaluated by analyzing cytotoxicity, cell cycle arrest and apoptosis in HepG2 cell line. Methods: Antibacterial activity was done by disc diffusion method and expressed as in millimeter diameter of zone inhibition. Antioxidant activity was done by ABTS radical assay, superoxide radical assay, iron chelation and uric acid formation inhibitory assays. The cytotoxicity efficacy was estimated using MTT assay. Annexin-V FITC kit was used to estimate the apoptosis and cell cycle arrest by flowcytometer. Morphological changes of cell through alteration of nuclear content and mitochondrial membrane potential were also examined using Hoechst and JC1 stains under fluorescence microscopy, respectively. Results: Highest antibacterial activity, TAA and RAA were found in EAF followed by DMF, HF and AF. Cytotoxicity of EAF was found to be 67% at 24 h and 83% at 48 h over the standard of quercetin (86% at 48 h). The cancer cells were found to be significantly (p < 0.05) higher in the proliferative G0/G1 phase where as significantly decreased in the S phase. Hence, treatment with T. conoides fraction showed statistically (p < 0.05) significant increase of apoptotic cells than that of quercetin standard (32%, 80 μg/mL). The apoptotic cell formation might be due to the change of nuclear content and mitochondrial membrane potential were further confirmed in HepG2 cells under fluorescence microscopy. Conclusion: Ethyl acetate fraction of T. conoides showed highest antibacterial, antioxidant and anticancer activity through exhibiting synergistic effects over the respective standard compounds.

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“…(Piracicaba, Braz. ), v.68, n.1, p.69-76, January/February 2011 are used to treat cough, asthma and diarrhea and the seeds for treatment of colic, jaundice and ophthalmic disorders (Behbahani et al, 2007b;Khan et al, 2000;MacKeen et al, 1997). The bark ethanol extract has antitumor property in mice (Khan et al, 2000), but the leaf ethanol extract displayed cytotoxicity against the HelA (human cervical carcinoma) cell lines with IC 50 value of 10 μg mL -1 (MacKeen et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Optimization of callus and cell suspension cultures of Barringtonia racemosa (Lecythidaceae family) for lycopene production

Behbahani

Shanehsazzadeh²,

Hessami

2011

Sci. agric. (Piracicaba, Braz.)

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Lycopene is present in a range of fresh fruits and vegetables, especially in the leaves of Barringtonia racemosa. The traditional lycopene extraction from the plant is being employed instead of an easy propagation technique like cell culture process from the leaf explants. We intend to assess how lycopene could be extracted via tissue culture under light (illuminance: 8,200 lux under white fluorescent lamps, photoperiod 16 h per day at 25°C) and dark. Leaf explants of Barringtonia racemosa were cultured on modified Murashige and Skoog (MS), Woody Plant Medium (WPM) and B5 media, supplemented with different concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D). Optimal conditions for callus induction and maintenance under both dark and light were investigated, and growth and lycopene accumulation were evaluated. Among media with different concentrations of 2,4-D, fast growing, friable callus initiated within three weeks after culturing on WPM basal medium supplemented with 2.0 mg L -1 (weight per volume) of 2,4-D, whereas callus induction in explants cultured on all other media started only after five weeks. Calli were subcultured once every fortnight. Pale yellow and green calli developed under conditions of dark and light respectively were then selected for evaluation of their lycopene contents. An improved reversed phase of high performance liquid chromatography (HPLC) method was used for a selective chemical determination of the lycopene content. Light induced lycopene production; and likewise maximum lycopene level incubated in light was higher than those incubated in darkness. The best growth rates of callus and cell suspension were achieved in WPM and B5 media respectively. The production of lycopene was growth-dependent through analysis of growth and lycopene content of both callus and cell suspension cultures. Key words: Powderpuff tree, carotene, phytochemical, plant micropropagation, growth hormones Otimização de culturas de suspensões de calos e células de Barringtonia racemosa (família Lecythidaceae) para produção de licopeno RESUMO: O licopeno está presente numa série de frutas frescas e hortaliças principalmente na folhas de Barringtonia racemosa. A extração tradicional do licopeno tem sido empregada no lugar da fácil técnica de propagação como o processo de cultura de células de explantes de folhas. É nossa intenção demonstrar como o licopeno pode ser extraído através de cultura de tecido sob luz (iluminação com lâmpadas fluorescentes brancas de 8.200 lux, 16 h por dia a 25° C) e escuro. Explantes de folhas de Barringtonia racemosa foram cultivados em meio modificado de Murashige e Skoog (MS) para plantas lenhosas e meio B5, suplementado com diferentes concentrações de ácido 2,4-Diclorofenoxiacético (2,4-D). Condições ótimas para indução e manutenção de calos sob luz e escuro foram investigadas e avaliados o crescimento e acumulo de licopeno. Entre meios com diferentes concentrações de 2,4 -D, calos friáveis de crescimento rápido tiveram início em três semanas após serem cultivados em...

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Antimicrobial and Cytotoxic Properties of Some Malaysian Traditional Vegetables (Ulam)

Abstract: Ethanol extracts of 19 Malaysian traditional vegetables (locally known as 'ulam')

Cited by 120 publications

References 6 publications

Characterization of Chemical Constituent and Antibacterial Activity of Honje Fruit Skin (Etlingera elatior)

Characterization of Chemical Constituent and Antibacterial Activity of Honje Fruit Skin (Etlingera elatior)

Antibacterial, antioxidant and anticancer properties of Turbinaria conoides (J. Agardh) Kuetz

Optimization of callus and cell suspension cultures of Barringtonia racemosa (Lecythidaceae family) for lycopene production

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