Introduction: 5-Flurouracil (5-FU) is considered the backbone of most of chemotherapy regimens, it has been established for the treatment of several neoplasms but one of its most common complications is oral mucositis including tongue epithelium.Aim: This study aimed to explore the effect of Platelet rich plasma (PRP) against the cytotoxicity induced by 5-Flurouracil in albino rats' tongue.Material & Methods: forty male Albino rats were used in this study, 10 rats were used as PRP donors and the other 30 rats were classified into 3 groups, 10 rats each: Group I: 60 mg/kg of saline was injected ip. on day zero, and other 40 mg/kg was injected on the second day. Group II: 60 mg/ kg of 5-FU was injected ip. on day zero, and other 40 mg/kg was injected on the second day. Group III: 60 mg/kg of 5-FU was injected ip. on day zero, and other 40 mg/kg was injected on the second day then after 24 hours, 1ml/rat/day of PRP was injected locally into the tongue mucosa for 3 weeks. The animals were sacrificed after 1 week of the second injection in group I and II and at end of third week of PRP injection in group III. specimens were prepared for histological examination and immunohistochemical staining for CD3.Results: Histological examination revealed significant improvement of mucosal tongue epithelium and connective tissue in group III samples when compared to group II, supported by immunohistochemical results of CD3. Conclusion:PRP has accelerating healing effect on tongue epithelium and connective tissue.

Introduction: Wound healing is a highly complicated process achieved through definitely programmed stages. Hyaluronic acid is a natural component present essentially in the skin and other connective tissues, it has been used in many medical fields for acceleration of wound healing.Aim: This study aimed to investigate the therapeutic effect of hyaluronic acid on healing of tongue ulcer in diabetic rat model. Material & Methods:Thirty male Albino rats were subjected to diabetes induction and after 1 week the animals were classified into 3 groups, Group I: rats were subjected to tongue ulcer and did not receive any treatment. Group II: rats were subjected to tongue ulcer and the ulcers were treated with 3 times/ day topical applications of commercially used 0.2% high molecular weight hyaluronic acid. Group III: rats were received 1-2 IU/ g/day of insulin NPH injected subcutaneous and then subjected to tongue ulcer after one week from the starting of insulin injection. The animals were sacrificed at 4 and 10 days after ulcer induction, specimens were prepared for histological examination and immunohistochemical staining for α-SMA.Results: Histological examination revealed better enhancement of ulcer healing and reepithelization together with reorganization of the epithelium and connective tissue in group II and III samples when compared to group I after 4 and 10 days of ulcer induction. Conclusion:oral application of hyaluronic acid gel has beneficial effects in accelerating wound healing and improving all phases of wound repair in tongue ulcers in rats.