Nitrogen-containing plasma polymerization is of considerable interest for tissue engineering due to their properties on cell adhesion and mesenchymal stem cells (MSCs) response. In this study, low-pressure RF plasma of acetylene and nitrogen was used to deposit nitrogen-containing plasma polymerized coatings on several substrates. Deposition kinetics and surface characteristics of coatings were investigated in terms of RF power and gas flow ratio. OES was used to monitor the plasma process and investigate the relation between the film structure and plasma species. Presence of several bonds and low concentration of amine functional groups were determined using FTIR and Colorimetric methods. Contact angle goniometry results indicated about 30% increase in surface hydrophilicity. Stability of coatings in air and two different liquid environments was examined by repeating surface free energy measurements. Deposited films exhibited acceptable stability during the storage duration. Surface roughness measured by AFM was found to decrease with growing concentration of nitrogen. The deposition rate increased with increasing RF power and decreased with growing concentration of nitrogen. Zeta potential measurements of coatings revealed the negative potential on the surface of the thin films. Temporary suppression of collagen X in the presence of plasma coatings was confirmed by RT-PCR results.

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This study involved 18 patients who received object relations therapy-an analytic therapy based on transference and potential space-after having been diagnosed with major depressive disorder via the clinician version of the Structured Clinical Interview for DSM-IV (First, Spitzer, Gibbon, & Williams, 1996). In-depth interviews were carried out to the point of saturation, after which the experiences of the patients were used as the basis for establishing an independent substantive theory. The findings were categorized into the following themes: context of therapy, barriers to therapy, facilitators of therapy, analytical dyad formation, the development of self-awareness and self-expression, and the realized self. Analyzing patients' experiences with therapy can play an important role in filling the conceptual gaps of object relations therapy and in identifying its therapeutic effects, which can expand its applicability to other disorders.