Introduction: several studies demonstrated the potential role of serum leptin in patients with premature ejaculation. Aim: we aimed in this study to evaluate the correlation between body mass index, serum leptin and intra-vaginal ejaculation latency time in patients with lifelong premature ejaculation and healthy controls. Patients and Methods: this study was carried out on 80 consecutive patients and controls. Body mass index was measured in the patients and the controls. Serum leptin was measured in both groups at the end of the study. Additionally, the patients and the controls were asked to measure intra vaginal ejaculation latency time (IELT) by stop watch handled by their wives for 2 successive months. Results: our prospective analysis revealed a highly significant association between body mass index and serum leptin and intra-vaginal ejaculation latency time among the cases (p-value < 0.001, <0.001 respectively). Additionally, body mass index (BMI) did reveal significant association with intra-vaginal ejaculation latency time (p-value=0.014) only, without any association with serum leptin (p-value = 0.391) among the controls. Discussion: the current study demonstrated a statistically significant association between BMI and serum leptin and IELT in the patients with lifelong premature ejaculation. Additionally, the results revealed a statistically significant association between BMI and IELT in the controls without any association with the serum leptin. Conclusion: the current study highlights the role of both body mass index and serum leptin as potential risk factors in patients with lifelong premature ejaculation. Eventually, future studies are required to duplicate this interesting association between body mass index, serum leptin and intra-vaginal ejaculation latency time in patients with lifelong premature ejaculation.

patients [8-11]. On the contrary, Höbarth et al. [12] pointed out that the prevalence of TM in patients who underwent US owing to a variety of scrotal symptoms was 0.6%. However, this incidence has increased in the past few years owing to technological advances and raising awareness about the condition [11]. In the same vein, Pedersen et al. [13] found recently that the prevalence of TM in patients who had an US investigation of the scrotum owing to testicular/scrotal symptoms was 12.8%. Moreover, Fedder [7] demonstrated that the prevalence rate of testicular hyperechoic foci (sonographic TML= testicular microlithiasis, FSH= follicle stimulating hormone, LH= luetinizing hormone) in nonvasectomized azoospermic males is 13.4%.

Background: DNA damage as Fragmentation has adverse effects on fertilization and embryo development, so it is one of the main causes of a male factor for infertility. Several techniques have been mentioned to elevation this damage. In our study, we determine DNA damage in human spermatozoa by sperm chromatin dispersion (SCD) method and Apoptosis of DNA in human spermatozoa by Optical density in gel electrophoresis in male infertility.Objects and Methods: Semen samples were collected from 100 men and were analyzed by standard light microscopic according to the World Organization (5 th edition) for diagnostic fertility. Furthermore, Sperm DNA damage was determined by using Halosperm Kit, then assessment apoptosis by optical density in Gel Electrophoresis. Results: The mean value of DNA by SCD method in infertile males increased with a value of 47.95±10.96 % when compared with the control value of 21.2 ±2.64 % with (p< 0.00001). On the other hand, the mean value of DNA by measurement of Optical density in Gel Electrophoresis in infertile males decreased with a value of 120.27±18.73 when compare with the control value of 144.4±45 with (p =0.833). Conclusion:The assessment of sperm DNA damage by SCD method and other methods for detection of DNA apoptosis by gel electrophoresis addition to routine semen analysis play important role in the diagnosis and management of male infertility.