The objective of this study was to screen Streptomyces spp. for biological control of root and stem rot (Sclerotium rolfsii) and bacterial wilt (Ralstonia solanacearum), the very destructive diseases of chili pepper in Thailand. About 265 isolates of Streptomyces spp. were tested for their inhibitory effects on S. rolfsii mycelial growth on dual culture plates. Then, 14 promising isolates were further tested for their effects on R. solanacearum growth. Three effective isolates further identified as S. mycarofaciens SS-2-243, S.philanthi RL-1-178 and S. philanthi RM-1-138 were selected and proved to produce both antifungal and antibacterial substances in the culture medium. S. philanthi RM-1-138 strongly inhibited seed germination and seedling growth of chili pepper in laboratory tests. Therefore, it was not used in the following studies. When tested in greenhouse conditions, the efficacy of S. philanthi RL-1-178 in suppressing Sclerotium root and stem rot of chili pepper was approximately equal to that of Trichoderma harzianum NR-1-52 or that of carboxin treatment. S. mycarofaciens SS-2-243 and S. philanthi RL-1-178 suppressed Ralstonia wilt of chili pepper in a way that was similar to streptomycin sulfate treatment and it was observed that T. harzianum NR-1-52 had no effect on the bacterial wilt. Under field conditions where the soil was inoculated with two pathogens, the results showed that S. philanthi RL-1-178 could protect the chili pepper plants from S. rolfsii and R. solanacearum infection better than S. mycarofaciens SS-2-243 or T. harzianum NR-1-52. S. philanthi RL-1-178 treatment resulted in 58.75% survival of chili pepper plants and its efficacy was not significantly different from the carboxin-andstreptomycin sulfate treatment.

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