In many applications, measured sensor data is meaningful only when the location of sensors is accurately known. Therefore, the localization accuracy is crucial. In this dissertation, both location estimation and location detection problems are considered.In location estimation problems, sensor nodes at known locations, called anchors, transmit signals to sensor nodes at unknown locations, called nodes, and use these transmissions to estimate the location of the nodes. Specifically, the location estimation in the presence of fading channels using time of arrival (TOA) measurements with narrowband communication signals is considered. Meanwhile, the Cramer-Rao lower bound (CRLB) for localization error under different assumptions is derived. Also, maximum likelihood estimators (MLEs) under these assumptions are derived.In large WSNs, distributed location estimation algorithms are more efficient than centralized algorithms. A sequential localization scheme, which is one of distributed location estimation algorithms, is considered. Also, different localization methods, such as TOA, received signal strength (RSS), time difference of arrival (TDOA), direction of arrival (DOA), and large aperture array (LAA) are compared under different signal-to-noise ratio (SNR) conditions. Simulation results show that DOA is the preferred scheme at the low SNR regime and the LAA localization algorithm provides better performance for network discovery at high SNRs. Meanwhile, the CRLB for the localization error using the TOA method is also derived.A distributed location detection scheme, which allows each anchor to make a decision as to whether a node is active or not is proposed. Once an anchor makes a decision, a bit is transmitted to a fusion center (FC). The fusion center combines all the decisions and uses a design parameter K to make the final decision. Three scenarios are considered in this dissertation. Firstly, location detection at a known location is considered. Secondly, detecting a node in a known region is considered. Thirdly, location detection in the presence of fading is considered. The optimal thresholds are derived and the total probability of false alarm and detection under different scenarios are derived.

We present a model-based clustering method, SWIFT (Scalable Weighted Iterative Flow-clustering Technique), for digesting high-dimensional large-sized datasets obtained via modern flow cytometry into more compact representations that are well-suited for further automated or manual analysis. Key attributes of the method include the following: (a) the analysis is conducted in the multidimensional space retaining the semantics of the data, (b) an iterative weighted sampling procedure is utilized to maintain modest computational complexity and to retain discrimination of extremely small subpopulations (hundreds of cells from datasets containing tens of millions), and (c) a splitting and merging procedure is incorporated in the algorithm to preserve distinguishability between biologically distinct populations, while still providing a significant compaction relative to the original data. This article presents a detailed algorithmic description of SWIFT, outlining the application-driven motivations for the different design choices, a discussion of computational complexity of the different steps, and results obtained with SWIFT for synthetic data and relatively simple experimental data that allow validation of the desirable attributes. A companion paper (Part 2) highlights the use of SWIFT, in combination with additional computational tools, for more challenging biological problems. © 2014 The Authors. Published by Wiley Periodicals Inc.

Abstract-A new filter structure using admissible wavelet packet analysis is presented. These filters have the advantage of having frequency bands spacing similar to the Mel scale. Further wavelet packet transform's multiresolution capabilities are used to derive new sets of features, which are found to be superior to Mel frequency cepstral coefficients (MFCC) in unvoiced phoneme classification problem.

A multistage clustering and data processing method, SWIFT (detailed in a companion manuscript), has been developed to detect rare subpopulations in large, high-dimensional flow cytometry datasets. An iterative sampling procedure initially fits the data to multidimensional Gaussian distributions, then splitting and merging stages use a criterion of unimodality to optimize the detection of rare subpopulations, to converge on a consistent cluster number, and to describe non-Gaussian distributions. Probabilistic assignment of cells to clusters, visualization, and manipulation of clusters by their cluster medians, facilitate application of expert knowledge using standard flow cytometry programs. The dual problems of rigorously comparing similar complex samples, and enumerating absent or very rare cell subpopulations in negative controls, were solved by assigning cells in multiple samples to a cluster template derived from a single or combined sample. Comparison of antigen-stimulated and control human peripheral blood cell samples demonstrated that SWIFT could identify biologically significant subpopulations, such as rare cytokine-producing influenza-specific T cells. A sensitivity of better than one part per million was attained in very large samples. Results were highly consistent on biological replicates, yet the analysis was sensitive enough to show that multiple samples from the same subject were more similar than samples from different subjects. A companion manuscript (Part 1) details the algorithmic development of SWIFT. © 2014 The Authors. Published by Wiley Periodicals Inc.

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