No abstract

Advanced glycation end products (AGEs) represent a set of molecules that contribute directly to the initiation and aggravation of diseases associated with ageing. AGEs are produced by the reaction between reducing sugars (or α-dicarbonyl compounds), proteins, and amino acid residues. Previous in vitro methods using non-enzymatic procedures described in the literature require an incubation period of 1–3 weeks to generate AGEs. In this study, the reaction time for the formation of AGEs (48 and 3 h) was significantly reduced by adaptation of methods previously described in the literature and coupling them to the free radical generation system termed hypoxanthine/xanthine oxidase assay. The incorporation of this assay into the experimental system accelerated the production of AGEs as a result of the formation of reactive oxygen species (ROS), as shown by increased fluorescence. The capacity of different classes of chemical compounds (aminoguanidine, chlorogenic acid, rutin, and methanol extracts of Hancornia speciosa Gomes) to inhibit protein glycation by acting as scavenging agents of α-dicarbonyl species was evaluated. Aminoguanidine and, especially, rutin identified in the leaf extracts of H. speciosa Gomes showed a high capacity to act as scavengers of reactive carbonyl species RCS-trapping, resulting in the inhibition of AGEs formation.

No abstract

Resumo. O presente trabalho apresenta o resultado da vivência de estudantes do ensino médio da cidade de Quixadá-CE com atividades de aprendizagem cooperativa aplicadas ao ensino de química. Quatro técnicas distintas de aprendizagem cooperativa (jigsaw, método dos pares, fila cooperativa e teste cooperativo) foram empregadas como estratégias de ensino-aprendizagem. Os resultados obtidos foram analisados no que diz respeito à apreensão dos conteúdos e observou-se um ambiente menos competitivo e mais colaborativo entre os estudantes, contribuindo assim para o desenvolvimento de aprendizagem significativa. O trabalho inclui descrição das técnicas de aprendizagem cooperativa, apresentação de todo o percurso metodológico utilizado, bem como a avaliação dos resultados.

No abstract