Obesity is a complex metabolic disease, which is increasing worldwide. The reduction of dietary lipid intake is considered an interesting pathway to reduce fat absorption and to affect the chronic energy imbalance. In this study, zebrafish larvae were used to analyze effects of cyanobacteria on intestinal lipid absorption in vivo. In total, 263 fractions of a cyanobacterial library were screened for PED6 activity, a fluorescent reporter of intestinal lipases, and 11 fractions reduced PED6 activity > 30%. Toxicity was not observed for those fractions, considering mortality, malformations or digestive physiology (protease inhibition). Intestinal long-chain fatty acid uptake (C16) was reduced, but not short-chain fatty acid uptake (C5). Alteration of lipid classes by high-performance thin-layer chromatography (HPTLC) or lipid processing by fluorescent HPTLC was analyzed, and 2 fractions significantly reduced the whole-body triglyceride level. Bioactivity-guided feature-based molecular networking of LC-MS/MS data identified 14 significant bioactive mass peaks (p < 0.01, correlation > 0.95), which consisted of 3 known putative and 11 unknown compounds. All putatively identified compounds were known to be involved in lipid metabolism and obesity. Summarizing, some cyanobacterial strains repressed intestinal lipid absorption without any signs of toxicity and could be developed in the future as nutraceuticals to combat obesity.

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Abstract. Glass can be considered to be a high-technology engineering material with a multifunctional potential for structural applications. However, the conventional approach to the use of glass is often based only on its properties of transparency and isolation. It is thus highly appropriate and necessary to study the mechanical behaviour of this material and to develop adequate methods and models leading to its characterisation. It is evident that the great potential of growth for structural glass applications is an important opportunity of development for the glass industry and the building/construction sectors. The work presented in this paper is a reflection of this conclusion. The authors shortly present the state-of-the-art on the application of glass as a structural element in building and construction, and refer to other potential fields of application and available glass materials. The experimental procedures and methods adopted in three-point bending tests performed on 500 × 100 [mm 2 ] float, laminated and tempered glass specimens with thicknesses between 4 and 19 mm are thoroughly described. The authors evaluated the mechanical strength and stiffness of glass for structural applications. This work contributes to a deeper knowledge of the properties of this material.