Objectives. This study was undertaken to investigate the OPG profiles in gingival crevicular fluid (GCF), saliva, and gingival tissues of chronic periodontitis (CP) patients in response to open flap debridement (OFD). Subjects and Methods. The study included 30 subjects divided into 2 groups: 20 CP patients and 10 periodontally healthy subjects. Plaque index, gingival index, pocket depth, and clinical attachment level measurements were recorded for all subjects. GCF, salivary, and gingival samples were collected from all 30 subjects at baseline and 3 and 6 month after OFD from the 20 CP patients. GCF and salivary OPG levels were assessed by ELISA assay, while OPG expression in gingival tissues was examined by immunohistochemistry. Results. GCF, salivary and gingival OPG profiles were significantly higher in control subjects compared to CP patients at baseline (P < 0.001). Within CP group, OPG levels in GCF, saliva, and gingival samples showed a significant increase at 3 and 6 months after OFD (P < 0.001) compared to baseline. Although OPG values increased significantly in gingival samples and insignificantly in saliva after 3 months compared to 6 months, yet GCF levels were significantly decreased. Conclusions. OPG might be considered as a diagnostic and prognostic biomarker of periodontal bone destruction. This trial is registered with NCT02160613.

Objectives:This study was conducted to estimate, compare and correlate the serum and salivary vitamin D3 (VitD3) in form of [25 (OH) D] and calcium (Ca) levels in healthy subjects and chronic periodontitis (C.P) patients. Subjects and methods:The study included 50 subjects divided into 2 groups: 30 C.P patients and 20 periodontally healthy subjects. Clinical examination was performed for all subjects, salivary and blood samples were collected from all 50 subjects. Salivary and serum VitD3 levels were assessed by ELISA assay, while Ca was measured colouremetrically.Results : Salivary and serum VitD3 levels were significantly higher in control subjects compared to C.P patients. Salivary Ca level was significantly higher in C.P group than control group but serum Ca level showed insignificance higher level in control group than C.P patients. No significance correlation was observed except for saliva and serum VitD3 levels there was a statistical significance correlation in the control group. Conclusion:Low salivary and serum VitD3 and high salivary Ca levels are associated with periodontitis, they might be considered as risk factors for periodontal diseases but this association need to be confirmed in more future studies. , et al.: Plasma 25 hydroxy vitamin D concentrations and periodontal disease in postmenopausal women. J Periodontol.; 84: 1243-56, 2013