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Cellulose is the integral component of lignocellulosic biomass which is found abundantly in nature. The lignocellulosic bioconversion is an emerging technology which utilizes microbes and their enzymes for serving the scientific community. In this study, about 126 isolates were isolated from the termite mound soil among which 81 isolates produced cellulase enzyme. Three isolates B12, B25 and B64 exhibited maximum enzyme activity and chosen for optimization parameters such as temperature, pH and incubation time. The isolates B12, B25 and B64 were identified based on 16S rRNA sequencing as Mesobacillus jeotgali, Lysin, Bacillus fusiformis and Bacillus sp respectively. The temperature, pH and incubation time for maximum cellulase production was optimized from the range and depicted that maximum activity at 40 o C, 7 and 60 hours respectively.