Viewing the considerable potential of marine agar as a source for the sustainable production of energy as well as nature-derived pharmaceutics, this work investigated the catalytic activity of three novel GH50 agarases from the mesophilic marine bacterium Microbulbifer elongatus PORT2 isolated from Indonesian coastal seawaters. The GH50 agarases AgaA50, AgaB50, and AgaC50 were identified through genome analysis; the corresponding genes were cloned and expressed in Escherichia coli BL21 (DE3). All recombinant agarases hydrolyzed β-p-nitrophenyl galactopyranoside, indicating β-glycosidase characteristics. AgaA50 and AgaB50 were able to cleave diverse natural agar species derived from Indonesian agarophytes, indicating a promising tolerance of these enzymes for substrate modifications. All three GH50 agarases degraded agarose, albeit with remarkable diversity in their catalytic activity and mode of action. AgaA50 and AgaC50 exerted exolytic activity releasing differently sized neoagarobioses, while AgaB50 showed additional endolytic activity in dependence on the substrate size. Surprisingly, AgaA50 and AgaB50 revealed considerable thermostability, retaining over 75% activity after 1-h incubation at 50 °C. Considering the thermal properties of agar, this makes these enzymes promising candidates for industrial processing.
ABSTRAK Tingkat konsumsi sayur di kalangan masyarakat Indonesia masih tergolong rendah dan tidak mengalami peningkatan selama pandemi meskipun masyarakat menyadari pentingnya mengonsumsi sayuran bagi kesehatan. Kegiatan pengabdian masyarakat ini bertujuan untuk edukasi gaya hidup sehat yang dapat mencegah diri dari terserang penyakit kronis melalui kampanye “Selangkah Menuju Hidup Sehat dengan Jus Sayur”. Pelaksanaan kegiatan dilakukan terintegrasi dengan program kuliah kerja nyata (KKN) yang merupakan salah satu mata kuliah wajib mahasiswa Universitas Padjadjaran. Sasaran yang dijadikan sebagai target adalah masyarakat RW IX, perumahan Prima Harapan Regency, Bekasi. Metode pelaksanaan kegiatan dibagi menjadi tiga tahap utama, yaitu tahap persiapan, tahap pelaksanaan, dan tahap tindak lanjut. Tahap persiapan mencakup perancangan kegiatan, tahap pelaksanaan mencakup penyebaran kuesioner pra lapangan dan kampanye door to door, tahap tindak lanjut mencakup evaluasi kegiatan. Waktu pelaksanaan kegiatan adalah antara 7 Januari hingga 7 Februari 2022. Evaluasi kegiatan menunjukkan bahwa kegiatan kampanye berhasil dalam menjalankan programnya dengan terlaksananya edukasi healthy veggie juice kepada warga dalam upaya penerapan gaya hidup sehat. Seluruh warga yang menjadi target kampanye door to door menyambut baik tester jus sayur yang diberikan dan memberikan feedback yang positif terhadap rasa aneka jus tersebut. Kata Kunci: Kampanye, Jus Sayur, Penyakit Kronis, Gaya Hidup Sehat ABSTRACT The level of vegetable consumption among Indonesians is still relatively low and has not increased during the pandemic even though people are aware of the importance of consuming vegetables for health. This community service activity aims to educate healthy lifestyles that can prevent themselves from developing chronic diseases through the "Healthy Life with Vegetable Juices" campaign. The implementation of the activity was carried out in an integrated manner with the subject of field work experience which is one of the compulsory subjects for Padjadjaran University students. The subject was the people living in the community, housing area Prima Harapan Regency, in Bekasi city. The method of implementing the activities is divided into three main stages, namely the preparation stage, the implementation stage, and the follow-up stage. The preparation stage includes the design of activities, the implementation stage includes the distribution of pre-field questionnaires and door-to-door campaigns, the follow-up stage includes the evaluation of activities. The implementation time of the activity is between 7 January to 7 February 2022. The evaluation of the activity shows that the campaign activities have succeeded in carrying out the program by implementing healthy veggie juice education to residents in an effort to implement a healthy lifestyle. All people living in community who became the target of the door-to-door campaign welcomed the vegetable juice tester provided and gave positive feedback on the taste of the various juices. Keywords: Campaign, Vegetable Juice, Chronic Disease, Healthy Lifestyle
Salah satu sumber antropogenik polutan laut berpotensi bahaya berasal dari kosmetika berupa plastik kemasan, microbeaddan beberapa bahan aktif produk perawatan kulit. Menjaga dan melestarikan ekosistem laut dari ancaman polusi yang membahayakan memerlukan partisipasi aktif dari masyarakat dan menjadi salah satu indikator pembangunan berkelanjutan (SDG). Penelitian ini bertujuan untuk mengetahui level pengetahuan dan mengukur kesadaran masyarakat terhadap keberadaan produk eco-friendly skincare. Penelitian ini bersifat kualitatif menggunakan metode kuesioner dan hasil kuesioner diolah secara deskriptif.Hasil penelitian menunjukkan bahwa para responden telah memiliki pengetahuan yang baik tentang ekosistem laut khususnya terumbu karang serta mendukung keberadaan produk berekolabel. Namun, separuhnya belum mengetahui dampak negatif bahan bahan aktif skincare bagi ekosistem maupun organisme perairan. Aspek kehalalan, khasiat dan keamanan produk bagi kesehatan menjadi dasar utama responden dalam memilih suatu produk kosmetik dan skincare. Aspek pengetahuan tentang keamanan produk bagi lingkungan dan keterjangkauan atau daya saing harga dengan produk non eco-label diketahui mempengaruhi keputusan responden untuk mengutamakan pemilihan produk berekolabel
ABSTRAK Virtual konseling merupakan salah satu upaya guna meningkatkan kesejahteraan psikologis selama masa pandemi Covid-19, khususnya bagi anak dan remaja sebagai sasaran dalam kegiatan pengabdian kepada masyarakat yang bertema Who Am I. Kegiatan virtual konseling ini ditujukan mengenalkan teknik pengenalan diri, wellbeing therapy dan untuk mengetahui apakah kegiatan virtual konseling dapat meningkatkan pengetahuan dan keterampilan dasar anak dan remaja untuk memelihara kesejahteraan psikososial yang dimilikinya. Metode kegiatan ini adalah konseling secara virtual melalui platform zoom dengan memberikan materi, latihan, dan permainan yang disusun untuk menjadi katalis peningkatan kesejahteraan psikologis. Konsep teoretis yang mendasari kegiatan ini adalah kesejahteraan psikologis, self-concept, self-image, life-skill, dan juga privasi. Kegiatan ini memperlihatkan bahwa virtual konseling yang ditata materinya guna meningkatkan kesejahteraan psikologis mampu menjadi sarana edukasi awal bagi anak dan remaja untuk mengenali diri mereka sendiri. Latihan mandiri secara berkelanjutan perlu dilakukan para peserta agar pengetahuan yang didapat dari kegiatan dapat diolah menjadi keterampilan individu untuk memelihara dan meningkatkan kesejahteraan psikologis yang telah dimiliki. Salah satunya adalah dengan melakukan dan mendalami aspek keagamaan dalam kehidupan sehari-hari. Kata kunci: kesejahteraan psikologis, anak, remaja, pandemi Covid-19, virtual konseling ABSTRACT Virtual counseling is one of the efforts to improve psychological well-being during the Covid-19 pandemic, especially for children and adolescents as targets in community service activities with the theme of Who Am I. This virtual counseling activity aims to introduce self-awareness techniques, deliver well-being therapy, and determine whether virtual counseling can improve children and adolescents' basic knowledge and skills in maintaining their psychosocial well-being. The method of this activity is virtual counseling through the zoom platform by providing materials, exercises, and games that are designed to be a catalyst for increasing psychological well-being. This activity's theoretical concepts are psychological well-being, self-concept, self-image, life skills, and privacy. This activity showed that virtual counseling delivery using organized materials to improve psychological well-being could be a means of initial education for children and adolescents to identify themselves. Continuous independent training needs to be carried out by the participants so that the knowledge gained from the activities can be processed into individual skills to maintain and improve the psychological well-being that they already have, for example, by doing and exercising religious aspects in everyday life. Keywords: Psychological Well-being, Children, Adolescent, Covid-19 Pandemic, Virtual Counseling
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