The guinea pig (Cavia porcellus) is a small animal that provides a source of protein for the feeding of rural inhabitants of Peru. The frequency of pathogens associated with cervical lymphadenitis includes mainly pyogenic bacteria’s Streptococcus sp., Staphylococcus sp. and Corynebacterium sp. The spread of these pathogens can be controlled by applying biosecurity with thorough disinfection and rest of ponds or cages. In this context, antibiotic resistance is a growing problem in veterinary and human medicine, disfavoring antibiotic therapeutic efficacy that can nullify the curative action during treatment. We describe the main bacteria isolated from cervical lymphadenitis can be very sensitive to bacitracin, polymyxin, vancomycin and gentamicin. Which will allow the proper use of antibiotics in guinea pigs, as well as an efficient control focused in One World One Health. Antimicrobial resistance against lymphadenitis in guinea pigs in vivo has not been well studied in the regions of Peru. Resistance to enrofloxacin, penicillin and oxytetracycline, the most widely used antibiotics by producers, is suspected without considering the specificity or the spectrum of their pharmacological action; which could represent a relevant public health problem at the regional level. For these considerations we suggest carrying out resistance tests with animals previously administered with fluoroquinolones and the new beta-lactams.