No abstract

The profile and bioactivity of essential oil (EO) depends on genetic, environmental, and other factors. We hypothesized that the basil EO may be influenced by the distillation methods. Hence, a study was conducted to evaluate the effect of steam distillation (SD) and hydrodistillation (HD) extraction method on the yield, composition, and bioactivity of EO of sweet basil (Ocimum basilicum) and holy basil (Ocimum tenuiflorum). In both basil species, the EO yield (content) was significantly higher from SD than from HD. There were significant differences in the compounds' concentrations of EO obtained from SD and HD as well, however, the same compounds were identified in the EO from HD and SD. In the EO of O. basilicum, the concentration of 74% of the identified compounds were higher in SD than HD, whereas in the EO of O. tenuiflorum, the concentration of 84% of identified compounds were higher in SD than in HD. However, the concentrations of two of the major compounds of O. basilicum EO (estragole and methyl cinnamate) and a major compound of O. tenuiflorum EO (methyl eugenol) were significantly higher in HD than in SD. The type of distillation did not affect the antioxidant capacity of basil EO within the species. This study demonstrated that the type of distillation may significantly affect oil yield and composition but not the antioxidant capacity of the EO from sweet and holy basil.

Acidification of agricultural soils under intense management in the Palouse region of eastern Washington and northern Idaho is of increasing concern. Buffer methods can provide lime requirement estimates (LREs); however, locally calibrated methods are lacking. Our objective was to evaluate buffer methods and to determine which can produce optimal LREs for Palouse agricultural soils. Samples from 10 regionally dominant agricultural soils (initial pH ≤5.3) were assessed for pH changes after incubation with nine levels of calcium carbonate (CaCO 3) for 90 d under laboratory conditions. Achieving a target pH of 6 in the top 15 cm of the soil profile required 3.36-8.36 Mg ha −1 of CaCO 3. Laboratory incubation results were compared with LREs calculated from 10 established calibrations using data from seven buffer methods: