No abstract

No abstract

No abstract

Vertebrate DNA contains a small fraction of unmethylated CpG-rich DNA sequences, many of which include the 5' end of a gene. This fraction can be detected by its cleavage to tiny fragments with the methylation-sensitive restriction enzyme Hpall. Thus, the Isolation of Hpall tiny fragments (HTFs) from a specific chromosome region may be a useful approach for making an Inventory of the genes contained in it. Using microdissection, we have Isolated DNA from human chromosome band 8q24.1.The DNA was digested with Hpall, Iigated to a Clol-cut pUC plasmid, and amplified with Toq DNA polymerase and the standard M13/pUC forward and reverse sequencing primers. The amplification products were used to construct an HTF library, which is enriched for CpG-rich single-copy clones.