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The oxidative activities of hydroponically produced Chinese kale (Brassica oleracea var. alboglabra L.) seedlings grown under different concentrations of selenium (Se) biofortification with sodium selenite (Na2SeO3) at 0, 5, 10, 15, and 30 mg·Se/L and different ages (5, 10, and 15 days after germination) were determined by the electron transfer method (DPPH and FTC). Highest antioxidant activities of Se-enriched Chinese kale seedlings (Se-KS) were found in seedlings grown on 30 mg·Se/L, 15 days after germination. The physicochemical contents (moisture, crude fibre, ash, crude protein, and carbohydrate), total Se contents, and Se bioaccessibility and antioxidant activities (after treatment with an in vitro simulated gastrointestinal digestion process) between Se-KS and a control of regular Chinese kale seedlings (R-KS) were compared. The crude protein and total Se contents of the air-dried matter in Se-KS were higher (37 ± 1% and 433 ± 22 mg·Se/kg) than those of the R-KS (33 ± 2% and 17 ± 3 mg·Se/kg). Total Se content and antioxidant activities were strongly correlated in the range from 0.93 to 0.99. Testing acute toxicity, a single dose of the Se-KS was orally administrated to rats at 1250, 2500, and 5000 mg/kg·b.w., which consisted of 0.54, 1.08, and 2.16 mg Se/kg·b.w., respectively. These results demonstrated that Se-KS did not create any toxicological signs and mortality. No abnormal pathological changes in the liver, kidney, and heart were found. The results suggested that the Se-KS could be a good source of organo Se for development as a new Se supplementary product.

This study aimed to determine the effectiveness of Se from hydroponically produced Se-enriched kale sprout (HPSeKS) on productive performance, egg quality, and Se concentrations in egg and tissue of laying quails. Two-hundred quails, 63 days of age, were divided into four groups. Each group consisted of five replicates and each replicate had ten birds, according to a completely randomized design. The experiment lasted for 6 weeks. The dietary treatments were T1 (control diet), T2 (control diet plus 0.2 mg Se/kg from sodium selenite), T3 (control diet plus 0.2 mg Se/kg from Se-enriched yeast), T4 (control diet plus 0.2 mg Se/kg from HPSeKS). The findings revealed that productive performance and egg quality of quails were not altered (p > 0.05) by Se sources. Whole egg Se concentrations of quails fed Se from HPSeKS and Se-enriched yeast were higher (p < 0.05) than that of quails fed the control diet. Breast muscle Se concentrations in quails fed Se from HPSeKS were higher (p < 0.05) than that of quails fed Se from sodium selenite and Se-enriched yeast. Heart tissue Se concentrations of quails fed Se from Se-enriched yeast and HPSeKS were similar (p > 0.05), but higher (p < 0.05) than that of quails fed Se from sodium selenite. The results reveal that Se from HPSeKS did not change the performance and egg quality of quails. The effectiveness of Se from HPSeKS was comparable to that of Se-enriched yeast, which was higher than that of Se from sodium selenite.

An experiment was conducted to determine the efficacy of Se from hydroponically produced Se-enriched kale sprout (HPSeKS) on performance, carcass characteristics, tissue Se concentration, and physiological responses of broilers in comparison to that of Se from Se-enriched yeast and sodium selenite. Three hundred and sixty male broilers, 10 days of age, were assigned to 6 groups, 4 replicates of 15 broilers each, according to the completely randomized design. The dietary treatments were the following: T1: control diet; T2: control diet plus 0.3 mg Se/kg from sodium selenite; T3: control diet plus 0.3 mg Se/kg from Se-enriched yeast; and T4, T5, and T6: control diet plus 0.3, 1.0, and 2.0 mg Se/kg from HPSeKS, respectively. The results found that dietary Se supplementation did not (p > 0.05) alter performance and carcass characteristics of broilers. Se supplementation increased (p < 0.05) Se concentrations in the liver and kidney of broilers. Heart tissue Se concentration of broilers fed Se from sodium selenite was lower (p < 0.05) than that of broilers fed Se from HPSeKS and Se-enriched yeast. Selenium from HPSeKS increased higher (p < 0.05) GSH-Px activity when compared to Se from sodium selenite and Se-enriched yeast. The results indicated that the efficacy of Se from HPSeKS was comparable in increasing tissue Se concentration, but higher in improving GSH-Px activity in Rbc when compared to those of Se from Se-enriched yeast.