Background: Toxoplasmosis in pregnancy is associated with spontaneous abortions, low birth weight babies, congenital deformities and intrauterine deaths. In many developed countries, pregnant mothers are screened for infection with Toxoplasma gondii, the causative parasite, and treatment is offered early in order to prevent these complications. In most developing countries, including Khartoum, the burden and risk factors of T. gondii infection among pregnant women, thus making it difficult to plan and implement control measures.Study Objectives: This study aimed at determining the prevalence of T. gondii infections and their risk factors among pregnant women attending antenatal care clinics in Kigali, Rwanda.Methods: This was a cross-sectional descriptive study involving 384 pregnant women aged 18 years and above who were attending antenatal Omdurman friend ship hospital, between April 2015-May 2017.Venous blood samples were collected from study participants and screened for IgG and IgM antibodies against T. gondii using the ELISA technique. The participants were also interviewed about selected behaviors that predispose individuals to infections with T. gondii.Results: Out of the 300 surveyed pregnant women, 65(21.7%) serum samples were found to have IgG while IgM was showed only 3(1%) IgM. Surveyed populations were divided into four age groups: (up to 20 years), (21-30), (31-40 years) and (more than 40 years). The positive cases within each age group showed IgM in one (0.3%), one (0.3%), one (0.3%) and 0(0%) respectively. On the other hand IgG was detected in 9(3%), 36(12%), 19(6.3%) and one (0.3%) respectively. Exposure to cats has been considered a major risk factor for acquisition of infection [1]. In the present study, it appears that cats have no direct role in transmission of the disease and there is no stastistically association between cat contact and infection (P= 0.88 and 0.1 with IgM and IgG respectively).Also meat consumption has no role in disease transmission as no statistically association women with previous history of miscarriage in the study group showed T. gondii sero-prevalence rate of 42 and 3 using ELISA IgG and IgM respectively compared to 23 and 0 among others with no past history of miscarriage with no significant difference between them (P =0.76 and 0.18) respectively. Conclusion:The seroprevalence of T. gondii antibodies is relatively low among the pregnant women. Undercooked meat consumption and cat contact are not significantly associated with sero-prevalence of anti-T. gondii IgG and IgM among the pregnant women.

Background: There are different procedures for the diagnosis of the pregnant female suspected with toxoplasmosis, however time, cost, and accuracy of the test should meat patients need. Material and Method: Three hundered pregnant female collected from Saad Abualila hospital antenatal care unit, were undergo three different procedures for the diagnosis of Toxoplasma gondii infection. Toxolatex. Toxo IgG-IgM rapid test and ELISA were done for all pregnant female. The result described as frequency and percentage of positivity, also specificity and sensitivity of Toxolatex. Toxo IgG-IgM rapid test were assessed according to ELISA results.