Background: There are different procedures for the diagnosis of the pregnant female suspected with toxoplasmosis, however time, cost, and accuracy of the test should meat patients need. Material and Method: Three hundered pregnant female collected from Saad Abualila hospital antenatal care unit, were undergo three different procedures for the diagnosis of Toxoplasma gondii infection. Toxolatex. Toxo IgG-IgM rapid test and ELISA were done for all pregnant female. The result described as frequency and percentage of positivity, also specificity and sensitivity of Toxolatex. Toxo IgG-IgM rapid test were assessed according to ELISA results.