Modern biotechnology enables an organism to produce a totally new product which the organism does not or cannot produce normally through the incorporation of the technology of ‘Genetic engineering’. Biotechnology shows its technical merits and new development prospects in breeding of new plants varieties with high and stable yield, good quality, as well as stress tolerance and resistance. Some of the most prevailing problems faced in agricultural ecosystems could be solved with the introduction of transgenic crops incorporated with traits for insect pest resistance, herbicide tolerance and resistance to viral diseases. Plant biotechnology has gained importance in the recent past for increasing the quality and quantity of agricultural, horticultural, ornamental plants, and in manipulating the plants for improved agronomic performance. Recent developments in the genome sequencing will have far reaching implications for future agriculture. From this study, we can know that the developing world adopts these fast-changing technologies soon and harness their unprecedented potential for the future benefit of human being.

Rhizoctonia solani and Alternaria citri are major plant pathogens of citrus, causing considerable production losses. Chemical fungicides are widely used for disease control. Using the food poisoning technique under in vitro conditions, an experiment was undertaken to investigate the efficacy of several fungicides against those pathogens. To evaluate the effect on Rhizoctonia solani mycelial growth, five different chemicals, viz. SAAF (Carbendazim 12% WP + Mancozeb 63% WP), Bavistin (Carbendazim 50% WP), VACOMIL PLUS (Metalaxyl 15% WP + Copper oxychloride 35% WP), and Raze (Copper oxychloride 50% WP) were used at 100 ppm and 200 ppm concentration each. Similar chemicals were used for Alternaria citri except for additional Mancozab (Mancozeb 75% WP). Mycelial growth inhibition was measured until the fungus nearly covered the plate in control. All fungicides reduced the fungal growth compared to control. After 96 hours of incubation with Rhizoctonia solani, maximum inhibition (100%) was achieved at both concentrations of Bavistin, followed by SAAF @ 200 ppm (97.59%) and SAAF @ 100 ppm (88.25%), whereas VACOMIL PLUS and Raze had the minimum effect on the mycelial growth. Similarly, after 8 days of incubation of Alternaria citri, SAAF @ 200 ppm showed the highest inhibition (70.86%), followed by SAAF @ 100 ppm (65.11%), Mancozab @ 200 ppm (64.39%), and Mancozab @ 100 ppm (47.48%), but the effect of Bavistin, Raze, and VACOMIL PLUS had the lowest impact. The chemical proven effective against the pathogens should be trialed in pot and field experiments for further verification.

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