Sham ovariectomy (SOV) or ovariectomy (OVX) was performed at 0800h of metastrus; porcine serum (PS) or porcine follicular fluid (PFF) was injected intraperitoneally at 0830h and/or 1130h, with terminal blood samples collected at 1700h. Fluid from medium and large follicles in porcine ovaries, charcoal extracted to remove steroids, significantly reduced serum FSH, in a dose-dependent manner. Futhermore, the SOV rats were more sensitive to PFF with respect to serum FSH lowering than the OVX rats, although when enough PFF was injected, both groups showed the same level of suppression. Since serum LH was very low, the possible effect of PFF on this hormone could not be assessed.

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