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Biotic integrity assessment of a stream subject to acid mine drainage (AMD) indicated that a number of the expected taxa were absent. Acute and chronic toxicity assessment of water sampled in a stream subject to AMD impact indicate that even at near neutral pH the metal concentration in the water dominates the toxicity toward Daphnia pulex. Extending the observation period to the death of test cohort in an otherwise standard sub-chronic toxicity test, allowed for generating a life table for the D. pulex. The probability of population extinction was estimated from the statistical characteristics of a bootstrap estimation of the dominant eigenvalue of a Leslie matrix based on the life table. At the current concentration levels of metals, notably manganese and zinc, in the water, a high risk to aquatic life exists. It is suggested that the metal content of the stream water also be addressed in an AMD water treatment plan.

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With the increasing focus on environmental issues, the objective of this study is to evaluate the potential impact of contaminated sediments of the Blesbok Spruit near Witbank - which receives acid mine drainage (AMD) inter alia - on biota. Direct transfer of chemicals from sediments to organisms is considered to be a major route of exposure for many species, and therefore focusing attention on sediment contamination and highlighting the fact that sediments are an important resource. Acute toxicity tests were performed on Daphnia pulex using both extracted sediment interstitial water and surface water. Chemical analyses were also performed on the sediment, interstitial water and surface water samples. The toxicity results suggest that metal toxicity adds significantly to the toxicity of the stream water which is enhanced by the effect of pH. The pH of the stream and interstitial water was consistently below 4.5.

No abstract