Three‐dimensional (3D) printing technology is a rapid prototyping method that has recently been increasingly used in anatomy education. Magnetic resonance imaging, computed tomography, and 3D scanners are generally used to create 3D models. The aim of this study, which was performed without using the aforementioned devices, is to design sheep scapula models in a computer environment and compare bone models created with different filaments printed by a 3D printer with real bone. Photographs of sheep scapula were taken for modelling, and measurements were made from certain points. After the photographs were transferred to a computer environment, they were transformed into 3D using the Cinema 4D software, a computer‐aided design program. Models were created using a 3D printer employing polylactic acid (PLA) and thermoplastic polyurethane (TPU) filaments. By comparing the models created with PLA and TPU filaments to the real bone, it was found that they have a similar anatomical structure, with dimensional‐morphometric differences found at some points. It was also observed that the scapula model created with PLA filaments was more resistant to impacts than the real bone and that the model created with TPU filaments was more flexible, with very low fragility as compared to PLA and real bone. Therefore, this method allows obtaining a large number of durable models as an alternative to the real bone without the need for much manpower or equipment and without the need for a 3D reconstruction device.

In addition to classical anatomy applications, particularly in medicine education, the contribution of different methods (3D modeling, body painting and clay modeling) has been investigated for the purpose of anatomy training. In recent years, clay modeling which is the one of these methods is frequently used. The aim of the present study was to determine the contribution of clay modeling and plastic model dressing methods to veterinary anatomy training. The ruminant forelimb's bones, joints, muscles and nerves were chosen as the topic of this study. Clay material was used for bone models, coloured play dough were used for joints, and Colored Eva Sponges were used for muscles and nerves. Students were divided into two groups as new method group and classical method group. The students were given exams and the results of exams were evaluated at the end of each application. It was detected that the student success rate increased when clay modelling and plastic model dressing methods were employed (P<0.05). There was no significant difference for the lesson success rate between male and female students (P>0.05). With this study, the clay modeling and plastic model dressing methods applied for the first time in veterinary anatomy training was found to increase student achievement and motivation significantly. Furthermore, we believe that organ models prepared by this method will contribute to education.

Toxoplasmosis is a parasitic disease that is transmitted by a variety of routes, including the ingestion of raw or undercooked meat. It infects roughly one-third of the world's population and is caused by Toxoplasma gondii, an obligate intracellular parasite. The goal of this research is to detect the existence and genotypes of T. gondii in beef and mutton, two of the most widely consumed red meats in Gaza, Palestine, using both ELISA and PCR techniques. For this purpose, 60 red meat samples were collected from butcheries in Gaza city, during the period from January to March 2021. These samples were divided evenly between beef and mutton. This study found that beef is devoid of T. gondii, whether tested using ELISA or PCR. On the contrary, both approaches detected T. gondii in mutton; however, the percentage of positive samples reported differed. For example, whereas T. gondii was detected in 14 (46.66 %) of 30 samples using ELISA, only 5 (16.66 %) of positive samples were detected using PCR. The genotyping results of the current investigation showed that the three DNA isolates were T. gondii type II. A Chisquare test was also implemented to evaluate the prevalence of T. gondii and the type of red meat samples (mutton and beef) examined using PCR and ELISA. Similarly, in the detection of T. gondii, a comparison of the PCR approach and ELISA was conducted, and all of these relationships were shown to be statistically significant, with p values < 0.05. Meanwhile, this investigation found that beef samples were devoid of T. gondii infection. Regardless of whether it was examined with an ELISA or a PCR, this study revealed the occurrence of T. gondii in mutton. The current study also concluded that eating raw or undercooked mutton is a potential risk factor for the transmission of T. gondii infection to humans. Besides, the occurrence of T. gondii type II in the three genotyped ADNA isolates.

Sympathetic ganglions located in the cervical region are important organs that make the final synapse of the sympathetic nerve fibers reached to the head, neck, and forelimbs. As far as we know, there are not any anatomical data about cervical sympathetic ganglia in Saanen goat. In this study, we determined the nerve branches separated from the ganglia and the location of the ganglia. We also determined the expression of some enzymes and proteins such as tyrosine hydroxylase (TH), dopamine β-hydroxylase (DβH), neuropeptide Y (NPY) and substance P (SP) in ganglia. Ganglion cervicale craniale (GCC) was on the medial side of bulla tympanica. Mainly branches named as nn. carotici interni, n. jugularis and nn. carotici externi was found to be separated from this ganglion and thin branches joined to the nearby nerve. It was found that n. vertebralis, the two branches that constitute the ansa subclavian, and the thin nerve branches involved in the surrounding tissues and organs separated from ganglion cervicothoracicum (GCT) that located in the first intercostal space. A total of five ganglion cervicale medium (GCM) found at the junction of the two branches forming the ansa subclavia. Another ganglion was not found on where cervical part of truncus sympathicus in all dissections and histological examinations. DβH, TH, NPY and SP were revealed to be express in all ganglia. DβH and NPY in CCG, TH in MCG, DβH, NPY and TH in GCT were found to be more intense staining.

Telocytes, new interstitial cells that have received significant attention in recent years, have been detected in many organs, including the heart. The distinction between telocytes and other interstitial cells can only be made based on their ultrastructural characterization and immunophenotypic features. In this study, we examined the interstitial cells in the healthy heart tissues of Saanen goats to determine whether they are telocytes or not, by using a scanning electron microscope (SEM) and immunohistochemical and immunofluorescence staining methods. The SEM revealed oval and round telocytes with two to four telopodes. Some telopodes also had podoms. The staining for immunohistochemical and immunofluorescence methods used for CD34, c‐kit (CD117), and vimentin antibodies. Positive cells were detected in the heart muscle and heart valves by immunohistochemical staining. As these antigens can also be expressed by other non‐telocyte cells, we used double immunofluorescence staining with CD34/c‐kit and CD34/vimentin antibodies to identify true telocytes. Telocytes were determined in the right atrium and aortic valve. While telocytes were CD34+/c‐kit+ and CD34+/vimentin+, fibroblasts were CD34−/vimentin+. These results confirm the presence of telocytes in the hearts of Saanen goats.