P. communis L. (cv. Le-Conte) (pears) and M. domestica Borkh. (cv. Anna) (apples) are economic fruit crops cultivated in Egypt. Their leaves were assessed for their beta-sitosterol content and found to have 9.4 mg/ g dried leaves wt and 5 mg/g dried leaves, respectively. So we used the lipoidal leaves extracts in formulation of eight beta-sitosterol rich emulgels from which the most stable formulae were tested for their antimicrobial activity and finally, the formulae which exerted antimicrobial activity were biologically evaluated for wound healing against well known wound healing ointment Mebo ® which composed mainly of 0.25% betasitosterol in base of sesame oil and beeswax and wound contraction was statistically different in both formulae F3 and F8 from both control and Mebo ® groups which indicated better wound healing activity of these formulae ensured by further histopathological study of the healed wounds.

I N THIS work, Evaluation of the biological activity and the chemical composition of Mesembryanthemum nodiflorum and Aptenia cordifolia growing in Egypt were carried out. The chemical composition of both plants was evaluated by GC/MS and HPLC/MS analysis. GC/MS analysis of the unsaponifiable matter of Mesembryanthemum nodiflorum and Aptenia cordifolia resulted in the identification of 17 and 13 compounds; respectively, the major compounds were octadecane and neophytadiene; respectively. GC/MS analysis of the saponifiable matter of Mesembryanthemum nodiflorum and Aptenia cordifolia resulted in the identification of 12 and 18 compounds; respectively. The palmitic acid was the most abundant saturated fatty acid in both species. HPLC/MS analysis of Mesembryanthemum nodiflorum and Aptenia cordifolia resulted in the identification of 36 and 48 compounds; respectively. Finally biological studies indicated that the extract of Mesembryanthemum nodiflorum has more potent analgesic effect and also showed significant inhibitory activity against colon, cervix, liver and normal melanocyte carcinoma. The 70% ethanol extracts of the aerial parts of plants under investigation have a significant hypoglycemic effect, anti-oxidant, antiinflammatory and hepatoprotective activity; where Aptenia cordifolia extract was more potent than Mesembryanthemum nodiflorum extract.

Lipoidial profile of Ipomoea tricolor and Ipomoea fistulosa leaves were characterized by GC/MS led to the identification of 22 and 33 compounds represented (95.88 and 91.82 %), respectively, of I. tricolor and I. fistulosa unsaponifiable matter It is composed mainly of hydrocarbons and oxygenated compounds. The GC/MS of fatty acids methyl ester (FAME) led to the identification of 8 and 10 compounds for I. tricolor and I. fistulosa, respectively.Linolenic and palmatic acids were found as the predominant fatty acids. On the other hand, the petroleum ether extract of I.tricolor was fractionated on column chromatography to yield major compounds; 5-phenyldecane and 4-phenyldecane, as well as, triterpenes including β-amyrin, lupeol and simiarenol,in addition to αamyrin, β-sitosterol, stigmasterol and αamyrin acetate which isolated for the first time from this species.The petroleum ether extracts of I. tricolorand I. fistulosa showed a significant increase in DPPHinhibition activity at 0.01 and 0.05 ug/ml, respectively. Furthermore, I.tricolor extract exhibited a significant increase in cholinesterase inhibition activity at concentration 20 ug/ml and a significant anti-inflammatory activity with IC50 117.4µg/ml. It can be concluded that pet.ether extracts of I.tricolor and I.fistulosa at different concentrations have significant antioxidant, anticholinesterase and antiinflammatory activities related to various bioactive identified compounds.