The hospital is a workplace that has a high risk to the safety and health of human resources (HR) in the hospital. Control of sources and potential hazards in hospitals can be done with the use of personal protective equipment (PPE). PPE consists of gloves, masks, face/eye protection, protective gowns, and leg protectors. The purpose of this study was to evaluate the use of PPE in nurses at Hospital X Bantul District. This study used a qualitative descriptive method with a case study design. Data collection uses interview guidelines with 5 informants and the observation of 30 health workers. The sampling technique uses purposive sampling. Evaluation of the level of PPE use in nurses in good categories namely gloves as many as 24 health workers (100%), masks 30 (100%), no activities were found with indications of the use of goggle, protective gown/apron 15 officers (100%), gaiters 15 officers (100%) and 15 nurses (100%) headcovers. This is supported by policies and SOPs for the use of PPE and guidelines for nursing actions, human resources (HR), namely nurses who have received socialization and training, hospital funds related to the availability of PPE and training, the availability of PPE according to indications of action and the existence of supervision conducted by IPCLN and PPI teams in Operation room unit, inpatient room, and CSSD. Conclusion of this research was found not all health workers have appropriate and compliant behavior in the use of PPE in each unit.