Orchids (Chattleya sp) is popular plant which is favorted by peoples and has high economic value. It's price Rp. 562,000/plant. Tissue culture technique is needed to produce this flower quickly than conventional technique. This study have aims to determine the effect of some medium concentration of organic sweet potato (Ipomoea batatas L) on growing Cattleya sp and for knowing the best of concentration on the growth of Chattleya sp. The method used was Completely Random Design with four of factors, that is: 0 g /L, 75 g/L, 150 g/L, and 300 g/L. The results were analyzed with Anova test and Duncan test multiple ranger test at the level of 5%. Each treatment was repeated six times. Parameters were observed are the number of new shoots, long leaf, number of leaves, leaf width, long roots, and height of the plant for 5 weeks. Based on the results of analysis showed that the sweet potato extract on 150 g/L concentration give the best results in long root parameter, the concentration of 75 g/L gived the best results to long leaf parameter and concentration of 300 g/L gived the best effect against leaf width. Same concentrations of sweet potato did not give significantly effect to number of new shoots, plantlets height, and number of leaves.

Escherichia coli is a type of Gram-negative bacteria that is commonly found in the human digestive tract. It has been reported as one of the bacteria that has been resistant to several antibiotics. This study aims to determine four (4) types of weed extracts that are considered but have not been used as antibacterial compounds against E. coli. This experimental study used a completely randomized design with several types of plant extracts, namely Acalypha indica L., Ageratum conyzoides, Phyllanthus niruri L., and Amaranthus spinosios at various concentrations (0, 50, and 100%). The results showed that the plant extract of A. indica L. had the ability as an antibacterial against the growth of E. coli at concentrations of 50% (1.41 ± 0.12) and 100% (1.53 ± 0.01) compared to other extracts. Meanwhile, the lowest average diameter of the inhibition zone for E. coli bacteria was found in the treatment of A. spinosios grass leaf extract 50% (1.17 ± 0.05).

ABSTRAKTanaman tebu adalah keluarga poace yang berpotensi menghasilkan nilai ekonomi tinggi karena manfaatnya sebagai produk unggulan dalam menghasilkan gula serta bioetanol yang banyak digunakan oleh masyarakat luas. Untuk itu perlu upaya pemuliaan tanaman yang cepat untuk memenuhi kebutuhan pasar. Teknik kultur mikrospora merupakan aplikasi bioteknologi yang efisien dalam menghasilkan tanaman haploid. Teknik ini belum berhasil diaplikasikan pada tanaman tebu sehingga dalam penelitian ini bertujuan untuk memicu embriogenesis mikrospora tebu dengan aplikasi kultur mikrospora pada varietas bululawang. Metode yang digunakan yaitu dengan stress perlakukan perendaman mannitol 0.3M sebelum isolasi kultur pada lama perendaman yang berbeda. Analisis data menggunakan uji anova dan uji duncan. Hasil yang diperoleh yaitu Perlakuan stress yang diberikan berhasil memicu embriogenesis mikrospora dengan lama perendaman anther optimum pada 7 hari pada mannitol 0.3M Kata kunci : mikrospora uninukleat, mannitol, tanaman tebu ABSTRACT Sugar cane is a poace family that has the potential to produce high economic value because of its benefits as a superior product in producing sugar and bioethanol which is widely used by the wider community. For this reason, it is necessary to initiate plants that are fast to meet market needs. Microspora culture technique is an efficient application of biotechnology in producing haploid plants. This technique has not been successfully applied to sugarcane plants so that in this study aims to trigger microsporee sugarcane embryogenesis by application of microspore culture in Bululawang variety. The method used is stress treatment of mannitol 0.3M soaking before culture isolation at different soaking times. Data analysis using anova test and duncan test. The results obtained are stress treatments given successfully trigger microspore embryogenesis with optimum anther treatment time at 7 days in mannitol 0.3M

Indigenous fungi are fungi that can degrade organic compounds and make them a source of nutrition for metabolism and life so that they can to survive in various environments including environments polluted by ammonia waste from the rubber factory industry. This study was conducted to obtain fungal isolates that can survive in river water contaminated with ammonia from rubber industry waste in Jember. Isolation was obtained from river water contaminated with rubber factory waste containing ammonia and isolated using the media of Potatoes Dextrose Agar (PDA). The method used in this research is descriptive exploration, namely isolating and culturing fungi using the dilution method. The results of the isolation will identify the genus of fungi based on macroscopic and microscopic morphological characters. Data analysis was done descriptively. The results obtained 4 isolates of indigenous fungi that can degrade ammonia, namely Aspergillus sp., Fusarium sp., Penicillium sp., and Yeast groups.

Broccoli is a high value vegetable crop in Indonesia, however production is low due to limited number of suitable cultivars, so, breeding hybrid broccoli for warm climate is important.  The first step in hybridization is providing homozygote parent plants which can be done efficiently via microspore culture.  The objectives of this study were to determine 1) bud size that produce uninucleate microspore stage appropriate for culture; 2) pollen viability, 3) microspore development, in three broccoli cultivars (‘BL 10001’, ‘Royal Green’ and ‘Green Magic’). Various bud size (1 – 5 cm) was squashed and observed microscopically to determine bud size containing uninucleate microspore.  Pollen viability was determined by IKI staining and pollen germination method.  Chromosome number was counted on root tips using squash method with aceto-orcein stain. Various heat treatment schemes were conducted to induce microspreo development.  Result showed uninucleate microspore derived from 2-3 mm and 3-4 mm bud length of ‘BL-10001’ and ‘Royal Green’ was responsive for microspore development in culture.  Pollen viability varied among cultivars, 78-87% on IKI method and 15-16% on germination test. Microspore culture showed different embryogenesis response; pollen-like structure was produced by ‘BL 10001’.