It has been documented previously that defects in the generation of C(18)-ceramide, a product of ceramide synthase 1 (CerS1), also known as longevity assurance gene 1 (hLASS1), play important roles in the pathogenesis and/or progression of HNSCC. However, whether altered levels of ceramide generation in HNSCC tumors have any clinical relevance remains unknown. In this study, the levels of endogenous ceramides were measured in tumor tissues of 45 HNSCC patients as compared to their normal tissues using high-pressure liquid chromatography/mass spectrometry (LC/MS), and then possible link between ceramide levels and the clinical parameters of HNSCC were examined. The data showed that the levels of C(16)-, C(24)-, C(24:1)-ceramides were significantly elevated in the majority of tumor tissues compared to their normal tissues, while the levels of only C(18)-ceramide were significantly decreased in HNSCC tumors, especially in tumor tissues of male patients. Importantly, it was also shown here that decreased C(18)-ceramide levels in HNSCC tumor tissues were significantly associated with the higher incidences of lymphovascular invasion, and pathologic nodal metastasis. Importantly, attenuation of C(18)-ceramide was also positively linked to the higher overall stages of the primary HNSCC tumors. Therefore, these data suggest, for the first time, that the defects in the generation/accumulation of C(18)-ceramide might have important clinical roles in HNSCC, especially in lymphovascular invasion and nodal disease.

Introduction: Tuberculosis of the parotid gland is a rare clinical entity which causes some difficulties in diagnosis because of the similarities in presentation to that of a neoplasm. Diagnosis mainly relies in the treating physician having a high index of suspicion. The diagnosis is generally overlooked by otolaryngologists and most cases are undergoing unnecessary surgery.

Background/aim: The aim of this study was to validate the Turkish Nasal Obstruction Symptom Evaluation (T-NOSE) scale. Materials and methods:The NOSE scale was translated into Turkish. A prospective study was conducted involving adult subjects with nasal obstruction and a control group. The patients were divided into three groups, namely nasal septum deviation (NSD), septoplasty, and control groups. Internal consistency, test-retest reliability, validity, responsiveness, and the magnitude of the effect of surgery were all investigated.Results: In total, 253 subjects were enrolled in the study. Cronbach's alpha was 0.938 and 0.942 upon test and retest, respectively, which proved good internal consistency. The mean kappa value was 0.82, indicating a high level of reproducibility. The difference between postoperative and control groups was not statistically significant (P < 0.05). The T-NOSE score of the NSD group was 65.67 ± 16.77, while it was 10.75 ± 12.25 for the control group (P < 0.01). The mean score improved following septoplasty (P < 0.001). The magnitude of the effect of surgery was considered high. The correlation between the visual analogue scale and NOSE scores was 0.948. Conclusion:The T-NOSE scale is a valid instrument with good internal consistency, reliability, reproducibility, validity, and responsiveness.

These results suggest that laryngeal squamous cell carcinomas with high levels of fascin expression may be more aggressive than those with low expression levels. Further studies with larger series are needed to support these results and to clarify rationales.