Abstract-The thermostability and density of water-salt solutions of DNA, irradiated by non thermal coherent millimeter electromagnetic waves with frequency 64.5 GHz have been investigated using the methods of spectrophotometry and densitometry. It is shown that the thermostability of DNA and density of its solutions are increased, depending on time of irradiation. It is expected that under the influence of millimeter electromagnetic radiation the hydration of DNA and ions of Na + that are present in solution decrease. As a result, the physicochemical characteristics of DNA are changed.

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Thermostability of DNA complexes with anti-tumorous compound — mitoxantrone — at 0.011[Formula: see text]M and 0.11 M NaCl ionic strengths of solution by spectrophotometric and microcalorimetric methods has been studied. It was shown that at small fillings when one molecule of mitoxantrone corresponds to 250 or more base pairs of DNA, the thermostability of complexes strongly depends on the solution’s ionic strength; moreover, at an ionic strength of 0.011 M NaCl the melting temperature dependence on mitoxantrone concentration passes through a minimum. In the mentioned region of mitoxantrone concentration the melting enthalpy of the complexes increases linearly with mitoxantrone concentration enhancement. The observed phenomenon, which is not observed for ethidium bromide complexes with DNA, is qualitatively explained by the increasing of coil-shaped state of DNA–mitoxantrone complex due to additional freedom of rotation of mitoxantrone side groups.

In the present work, the thermostabilities of mitoxantrone (MTX) complexes with DNA from sarcoma 45 and healthy rat liver were studied. DNAs from both sources were irradiated by resonant (64.5 GHz and 50.3 GHz) and nonresonant (48.3 GHz) frequencies of water. The obtained data showed that DNA solution irradiation by resonant frequencies of water induces a dehydration of nucleotides and Na[Formula: see text] ions in the solution. It is shown that at relatively low concentrations of MTX, when one MTX molecule binds to almost 100 pairs of bases of DNA, the thermostabilities of complexes decrease. Moreover, this change is more pronounced ([Formula: see text]C) at the complex formation with DNA released from sarcoma 45 tumor.

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