-The objectives of this study were to estimate the crossability rate in combinations of and assess the occurrence of incompatibility barriers between Capsicum species. The species C. annuum, C. chinense, C. frutescens, C. baccatum var. pendulum, and C. baccatum var. baccatum were crossed and the number of hand-pollinations and of resulting fruits and plants was registered.
The resulting hybrids were evaluated for their pollen viability and in the crosses that produced no hybrids, the in vivo germination of pollen grains was assessed. Some crosses generated hybrids, while others produced no fruit or, despite generating fruits, no plants grew from them. The crossability rate between species of the complex C. annuum and C. baccatum ranged from 2.2% to 3.7%, and was 14.6% between species of the complex C. annuum. A pre-fertilization barrier was observed in the non-germination of pollen grains, as well as post-fertilization barriers consisting of embryo death, lack of vigor and hybrid sterility
ABSTRACT. Cajazeira (Spondias mombin L.), of the family Anacardiaceae, is a species of fruit tree found in the Amazon region with fruits that have excellent prospects for commercial use. We aimed to evaluate the genetic diversity within and among natural populations of S. mombin, with natural occurrence in northern Mato Grosso State, by using inter-simple sequence repeat (ISSR) markers. Overall, 126 individuals were evaluated from three populations located Alta Floresta (AFL) 42, Marcelândia (MAR) 41, and Nova Bandeirantes (NBA) 43. The individuals were genotyped with 14 ISSR primers, which amplified 99 fragments. All markers, with the exception of DiGA3'A, presented a polymorphic information content above 0.25, and thus, are Analysis of molecular variance showed that 77.38% of the total genetic variation is found within populations while 22.62% is found among populations. AFL and NBA are genetically the most similar populations and also the closest "Structure" revealed genetic diversity among the genotypes of each population. As there is genetic variability in both populations, and there are no genetically identical individuals, both populations can be a source of genotypes for germplasm banks and for future commercial fruitful plantations S. mombin.
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