RESUMO: Um Banco de Dentes Humanos (BDH) é uma instituição sem fins lucrativos, vinculada a uma faculdade, universidade ou outra instituição. Seu propósito é suprir as necessidades acadêmicas, fornecendo dentes humanos para pesquisa ou para treinamento laboratorial pré-clínico dos alunos, dessa forma eliminando o comércio ilegal de dentes que ainda existe nas faculdades de Odontologia. Cabe também ao BDH zelar pela eliminação da infecção cruzada que existe no manuseio indiscriminado de dentes extraídos. Para o bom funcionamento de um BDH, é fun damen tal um controle severo de seus procedimentos internos, que incluem a separação e o estoque de dentes, assim como o cadastro e o arquivamento das fichas dos doadores ou beneficiários. Aqui, abordaremos as funções que um BDH pode desempenhar e a forma pela qual hoje o Banco de Dentes Humanos da Faculdade de Odontologia da Universidade de São Paulo (FOUSP) funciona e organiza-se. DESCRITORES:Ban co de den tes hu ma nos; Orga ni za ção e ad mi nis tra ção.ABSTRACT: A Hu man Te eth Bank (HTB) is a non pro fit ins ti tu ti on, as so ci a ted to a col le ge, to a u ni ver sity or to some other ins ti tu ti on. Its pur po se is to ful fill aca de mic ne eds, by sup plying hu man te eth for re se arch or for pre cli ni cal la bo ra tory tra i ning of stu dents, thus eli mi na ting the il le gal com mer ce of te eth that still ta kes pla ce in den tal scho ols. It is also up to an HTB to work for the eli mi na ti on of cross-in fec ti on ca u sed by in dis cri mi na te hand ling of ex trac ted te eth. In or der to work pro perly, an HTB should have a strict con trol of its in ter nal pro ce du res, which in c lu de se pa ra ti on and stoc king of te eth, as well as an ap pro pri a te ma in ta i ning of do nors' and be ne fi ci a ri es' re cords. In this ar ti cle, we dis cuss the func ti ons that an HTB can per form, and how the Hu man Te eth Bank, Scho ol of Den tistry, Uni ver sity of São Pa u lo, works and or ga ni zes it self to day. DESCRIPTORS:Hu man teeth bank; Or ga ni za tion and ad min is tra tion.Um Ban co de Den tes Hu ma nos (BDH) é uma ins ti tu i ção sem fins lu cra ti vos, que deve es tar vincu la da a uma fa cul da de, uni ver si da de ou ou tra ins ti tu i ção. Seu pro pó si to é su prir as ne ces si da des aca dê mi cas, for ne cen do den tes hu ma nos para pes qui sa ou ati vi da des di dá ti cas.Den tro des se con tex to, a ins ti tu i ção BDH pas sa a as su mir im por tan te fun ção éti ca, eli mi nan do o co mér cio ile gal de den tes que ain da exis te nas facul da des de Odon to lo gia. De fato, o den te é um ór -gão do cor po hu ma no e, como tal, está sub me ti do à Lei de Trans plan tes Bra si le i ra (lei 9434 de 04/02/1997), a qual pre vê pena de 3 a 8 anos de re clu são e mul ta para quem re mo ver, post-mor tem, ór gãos, te ci dos e par tes do cor po hu ma no de pesso as não iden ti fi ca das. O Có di go Pe nal tam bém pre vê pena de 1 a 3 anos de re clu são para aque les que vi o la rem se pul tu ra (Arti go 210) e o Con se lho Na ci o nal de ...

Considering the improvement of biomaterials that facilitate atraumatic restorative techniques in dentistry, a papain-based gel can be used in the chemomechanical removal of decayed dental tissue. However, there is no information regarding the influence of this gel on the structure of sound collagen. The aim of the present study was to investigate the adsorption of a papain-based gel (PapacarieTM) to collagen and determine collagen integrity after treatment. A pilot study was first performed with 10 samples of type I collagen membrane obtained from bovine Achilles deep tendon to compare the influence of hydration (Milli-Q water) on infrared bands of collagen. In a further experiment, 10 samples of type I collagen membrane were used to evaluate the effects of PapacarieTM on the collagen microstructure. All analyses were performed using the attenuated total reflectance technique of Fourier transform infrared (ATR-FTIR). The results demonstrated that the application of PapacarieTM does not lead to the degradation of collagen and this product can be safely used in minimally invasive dentistry. As the integrity of sound collagen is preserved after the application of the papain-based gel, this product is indicated for the selective removal of infected dentin, leaving the affected dentin intact and capable of re-mineralization.

2012Effect of dental tissue conditioners and matrix metalloproteinase inhibitors on type I collagen microstructure analyzed by Fourier transform infrared spectroscopy JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, MALDEN, v. 100B, n. 4, pp. 1009-1016, MAY, 2012 Abstract: This study aimed to evaluate the chemical interaction of collagen with some substances usually applied in dental treatments to increase the durability of adhesive restorations to dentin. Initially, the similarity between human dentin collagen and type I collagen obtained from commercial bovine membranes of Achilles deep tendon was compared by the Attenuated Total Reflectance technique of Fourier Transform Infrared (ATR-FTIR) spectroscopy. Finally, the effects of application of 35% phosphoric acid, 0.1M ethylenediaminetetraacetic acid (EDTA), 2% chlorhexidine, and 6.5% proanthocyanidin solution on microstructure of collagen and in the integrity of its triple helix were also evaluated by ATR-FTIR. It was observed that the commercial type I collagen can be used as an efficient substitute for demineralized human dentin in studies that use spectroscopy analysis. The 35% phosphoric acid significantly altered the organic content of amides, proline and hydroxyproline of type I collagen. The surface treatment with 0.1M EDTA, 2% chlorhexidine, or 6.5% proanthocyanidin did not promote deleterious structural changes to the collagen triple helix. The application of 6.5% proanthocyanidin on collagen promoted hydrogen bond formation.

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The aim of this study was to investigate whether distinct cooling of low fluence erbium, chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser irradiation would influence adhesion. Main factors tested were: substrates (two), irradiation conditions (three), and adhesives (three). A 750 microm diameter tip was used, for 50 s, 1 mm from the surface, with a 0.25 W power output, 20 Hz, energy density of 2.8 J/cm(2) with energy per pulse of 12.5 mJ. When applied, water delivery rate was 11 ml/min. The analysis of variance (ANOVA) showed that laser conditioning significantly decreased the bond strength of all adhesive systems applied on enamel. On dentin, laser conditioning significantly reduced bond strength of etch-and-rinse and one-step self-etch systems; however, laser irradiation under water cooling did not alter bonding of two-step self-etching. It may be concluded that the irradiation with Er,Cr:YSGG laser at 2.8 J/cm(2) with water coolant was responsible for a better adhesion to dentin, while enamel irradiation reduced bond strength, irrespective of cooling conditions.