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BACKGROUND: A sensitive nanostructured immunoelectrode based on poly(allylamine) (PAH) sandwich is developed for nonstructural 1 (NS1) of dengue virus. NS1 is a secretory protein abundant in the acute phase of disease associated to hemorrhagic fever. Anti-NS1 antibodies are immobilized on the electrode surface by a thin layer of PAH assembled on carboxylated carbon nanotubes (CNTs). PAH is cationic polymer acting as bi-functional agent to tightly attach CNTs to the electrode surface and anti-NS1 antibodies through their Fc terminal, avoiding random immobilization. Electrochemical responses of immunoassay are generated at a controlled potential by a reaction between H 2 O 2 and peroxidase enzyme conjugated to anti-NS1 antibodies.