Alfalfa witches' broom (AWB) is one of the most important and destructive diseases of alfalfa in Iran. Based on characteristic disease symptoms and direct and nested polymerase chain reactions, the status of AWB disease was evaluated in different growing areas of Iran. Restriction fragment length polymorphism was used to identify AWB disease associated phytoplasmas. Furthermore, infection rate, disease severity, death rate of infected plant in the summer and winter and overwintering of disease vector were assessed. Based on the results, AWB disease was reported on different alfalfa cultivars in Yazd,
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