Prazosin hydrochloride is the first developed selective antagonist for α1 — adrenoceptors, which is used as antihypertensive agent. Statins are used in the treatment of various types of hypercholesterolemia. In the present paper a simple, specific an accurate RP‐HPLC method was developed and validated for the simultaneous determination of prazosin, atorvastatin, rosuvastatin and simvastatin in active and in dosage formulations. A nucleosil 100‐10, C‐18, 10μ column having 250 × 4.6 mm i.d. in isocratic mode, with mobile phase containing methanol:water:acetonitrile (70:20:10) adjusted to pH 2.5 ± 0.02 using orthophosphoric acid. The flow rate was 1 mLmin−1 and effluents were monitored at 240 nm. The % recovery for all the drugs in formulations was found to be 94‐105%. The parameters such as accuracy (%RSD less than 2), precision (%RSD less than 2), linearity (>0.999) were found to be satisfactory. The presented method was applied without any interference of excepients for the determination of tablets. The proposed method due to its low LOQ, excellent accuracy, precision and selectivity could be used for routine quality control.

A simple and sensitive colorimetric spectrophotometric method based on application of chemical kinetics was produced and developed for the quantitative determination of 3-amino methyl hexanoic acid (pregabalin) in bulk and pharmaceutical preparations. Ninhydrin, a chromogenic agent was reacted with pregabalin at 70°C and the absorbance was measured at 575 nm and kinetic correlation was evaluated between absorbance and time. The initial-rate, rate-constant and fixed-time (80 min) methods were employed for the estimation of pregabalin in the range of 10-30 µg mL-1 which showed good correlation >0.9990 in all three cases with LOQ values ranging from 1.2-1.5 µg mL-1 and LOD values ranging from 0.76-0.95 µg mL-1. Method validation was carried out using ICH recommendations and the variables were evaluated and optimized. The newly developed method has useful applications to determine pregabalin in pharmaceutical formulation with good recovery (98.38 to 102.12 %t) and precision (RSD < 1.4 %). Standard free energy changes and association constant were found to be 1.212 × 10 3 and-4.747, respectively, as determined by the Benesi-Hildebrand equation.

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This study was conducted to assess the pharmacy students and graduateknowledge and awareness about pharmacovigilance & adverse drug reporting (ADR) systemin Karachi, Pakistan. Objectives: This study was designed to identify the trends, perception andapproaches of pharmacy students and graduate towards current scenarios of pharmacovigilanceand ADR contextual to our setting. Study Design: It was cross sectional, qualitative study.Setting: Pharmacy final year students and fresh graduates of two public and two private sectoruniversities were included in the study. Period: Data was collected between January to August,2015. Method: Relevant information was collected using questionnaire with 18 open ended and7 close ended questions. 400 final year participants and 150 fresh graduates were incorporatedin this survey. SPSS 20.0 was used to analyze the results and Percent, frequencies and meanscores were calculated for various outcomes. Results: Response rate of final year studentsand fresh graduates was found (97%, n = 388) and (88%, n= 132) respectively. Students levelof awareness about pharmacovigilance was found (54%, n =216) in final year students whilelittle higher rates were observed (78%, n=110) in graduates. Concept of pharmacovigilancegained through pharmacy curriculum was calculated 45% rated by final year students. Thepharmacovigilance knowledge mean score was found to be 2.368.5 and 2.886.3 for final yearstudents and fresh graduates respectively. 58% total respondents were aware with relationshipbetween the drug and the ADR. Conclusion: The results of this study demonstrate that pharmacystudents of final year in public and private sector universities of Pakistan are aware with somebasic knowledge of ADRs and pharmacovigilance, but it is a need of time to incorporate morecontents of such aspects in curriculum with some practical exposure that how to report ADRs.

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