Background: Biocompatible MIL-100 (Fe), a metal organic framework material, has recently attracted increasing attention in biomedical engineering. The high surface area, pore volume, and accessible Lewis acid sites make MIL-100 (Fe) a proper candidate for hydrophobic anticancer drug loading and storage. In this study, a novel investigation of cyclophosphamide (CP) -loaded MIL-100(Fe) (MIL-100(Fe)/CP) and a simulation of drug loading at a molecular level is presented. Methods: This research used a facile synthesis method to prepare MIL-100(Fe), which addresses the high temperature and pressure challenges of synthesis methods. MIL-100(Fe) and MIL-100(Fe)/CP were characterized using x-ray diffraction (XRD), Brunauer–Emmett–Teller (BET), Fourier transform infrared (FTIR), and field emission scanning electron microscopy (FESEM). Results: The carriers' drug loading and release behavior are determined by using UV-visible spectrophotometry. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is applied to examine the biocompatibility and the anticancer effect of MIL-100(Fe)/CP on the human breast cancer cell line (MCF-7). Conclusion: In vivo antitumor experiments and histological observation reveal inhibition properties of MIL-100(Fe)/CP on the tumor cells. MIL-100(Fe)/CP, with 37.41% drug payload, represents impressive antitumor activity.

The current study aimed to manufacture and characterization of three-component nanocomposites Hydroxyapatite Using Polarization Method.. The new work in this paper is an antibacterial test for triple coating which reduces the number of bacteria perceptibly. the antibacterial and corrosion behaviors were evaluated in comparison to uncoated samples by the electrochemical coating polarization test method. Tests such as X-ray diffraction and scanning electron microscope were used to characterize the coating. The X-ray diffraction test results showed that a three-component composite coating was created on the substrate in optimal coating conditions. The scanning electron microscope images revealed that the morphology of the formed coating surface was uniform, and its thickness was about 22 to 26.5 micrometers. The results suggested that coating thickness increased with the progression of coating time. Corrosion test outcomes indicated that employing the nanocomposite layer enhanced the corrosion resistance of the coating. Antibacterial test results suggested that the application of the nanocomposite coating effectively reduced the bacteria growth on the surface.

MIL-100(Fe) was synthesized under biofriendly conditions at room temperature and pressure using iron(II) chloride as the source of iron, and it was coated with chitosan (CS), a natural polysaccharide. In this study, we used a computational technique to predict the amount of drug loading in MIL-100(Fe) and MIL-100(Fe)/CS with molecular dynamics software LAMMPS. Powder X-ray diffraction analysis was conducted to characterize the chitosan-coated MIL-100(Fe) loaded with cyclophosphamide (MIL-100(Fe)/CS/CP). The drug loading and release processes were quantified using UV spectroscopy at 193 nm. The toxic effect of MIL-100(Fe)/CS/CP was determined on human breast cancer (MCF-7) cells. In vivo images and H&E analysis show inhibition properties of MIL-100(Fe)/CS/CP on tumor cells. The conducted research indicates that computational calculation provides a unique insight into the drug adsorption since a proper understanding of the atomic interaction of MIL-100(Fe)/CS with anticancer drugs is important for developing experimental investigations. The biocompatibility and anticancer properties of chitosan molecules enhanced the tumor inhibitory effect of the particles compared with the MIL-100(Fe)/CP and free cyclophosphamide treatments.