Background The insulin receptor substrate 1 ( IRS 1 ) is a critical factor in the signaling pathway for insulin, and mutations in this gene have been reported, which contribute to the ability to develop type 2 diabetes. The polymorphisms in the promoter region of C‐C motif chemokine receptor5 ( CCR 5 ) are also being studied as candidates for susceptibility to develop type 2 diabetes. The aim of the current study was to determine the relationship between IRS 1 and CCR 5 polymorphisms with type 2 diabetes in the Kurdistan population. Methods Genomic DNA was isolated from the blood by salt extraction method and the polymorphisms were examined using Restriction Fragment Length Polymorphism ( RFLP ) method. Results The results of current study indicated that the frequency of AA genotype in type 2 diabetic patients in both CCR 5 ( OR = 2.9, p = 0.04) and IRS 1 ( OR = 3.3, p = 0.036) were significantly more than controls. Conclusion According to the results of this study, the presence of AA genotype in both CCR 5 and IRS 1 is associated with type 2 diabetes. There was no significant association between AG or GG genotypes with type 2 diabetes.

Background: The polymorphisms of the C–C chemokine receptor type 5 ( CCR5 ) and the insulin receptor substrate 1 ( IRS 1) have been studied as candidates for the susceptibility to develop type 2 diabetes mellitus (T2DM). CCR5 is a chemokine receptor, and the polymorphisms in the promoter region of this receptor are being studied as candidates for the susceptibility to develop T2DM. Also, IRS 1 is a critical factor in the signaling pathway for insulin, and mutations in this gene have been reported, which contribute to the ability to develop T2DM. The aim of the current study was to determine the relationship between CCR5 (59029A/G) and IRS 1 (rs10498210) polymorphisms with T2DM in Sanandajian patients. Methods: Genomic DNA was isolated from 200 healthy individuals and 220 Kurdish T2DM patients by salt extraction method and the polymorphisms were examined by restriction fragment length polymorphism (RFLP) method and then the results were analyzed using Chi-square test. Results: The frequency of AA genotype in 220 Kurdish patients for both genes CCR5 (OR=1.9, P =0.02) and IRS1 (OR [95% CI]=2.62, P =0.02) were significantly more than controls. There was no significant association between AG or GG genotypes in with T2DM. Conclusion: The presence of AA homozygote alleles in both loci of IRS1 (rs10498210) and CCR5 (59029A/G) genes increased the risk of T2DM.

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