No abstract

The species were collected from 3 main hospitals in different regions in Baghdad, and presented by one hundred and seventy-four for various genders and ages. The collected sources were 60 samples of urine, 33 samples of wounds, 36 samples of burns, 45 sample of fecal material. A total 60 isolates were isolated and diagnosed according to microscopic, cultural and biochemical tests as well as Api – 20 E, VITEK2 Compact systems were performed as well as specific 16SrRNA gene was applied for molecular identification of Klebsiella pneumoniae isolates deposited in the National Center for Biotechnology Information (NCBI) (From LC314482.1 - LC314486.1). As a result of these tests a total of 13 Klebsiella pneumoniae isolates were isolated and identified. For the precise detection of studied isolates in molecular techniques, chromosomal DNA was extracted. It was clear that the percentage of VanA gene appearance was 40% and 6.66% VanB gene for the selected isolates. Vancomycin-resistant genes were investigated for these bacterial isolates using specific primers for the resistant genes (VanA & VanB). Results of gel electrophoresis of the PCR products for Klebsiella pneumoniae that they have the resistant genes (VanA & VanB) appeared on chromosomal DNA.

The objective of this study to evaluate the existence of Streptococcus equi subspecies equi as probable agents of naturally occurring infection of the equine upper respiratory disease from the Equestrian club in Baghdad city. Nasal swabs and pus samples from 141 horses with upper respiratory tract infections were collected. Results indicated that different microorganisms were isolated and identified S. equi subsp equi (30 isolates), S. equi subsp zooepidemicus (14 isolates), S. equisimilus (9 isolates), Enterococcus. fecalis (17 isolates), Pasteurella spp. (29 isolates), Staphylococcus spp. (25 isolates), Bacillus spp. (24 isolates), Pseudomonas spp.(16 isolates), and E. coli (21 isolates). All 30 isolates of S. equi was characterized by biochemical tests. For molecular identification of the subspecies S. equi one genomic region SeM was amplified.