Buffalo semen collected from Murrah bull were cryopreserved and evaluated for different motility parameter, kinematics and plasma membrane integrity. Buffalo bulls were maintained uniform standard management and nutritional practices. Semen was collected regularly twice a week semen collection schedule from four (04) Murrah bull. Collected semen was immediately transported to laboratory and evaluated for different macroscopic parameter (color, volume and thickness). Fresh semen was then diluted with saline solution and evaluated for sperm concentration, motility, sperm kinematics and morphology. Semen samples that fill all the standard were selected for freezing and diluted with Tris-egg yolk citrate diluter. Diluted semen was equilibrated, cryopreserved and finally evaluated for post thaw sperm quality. Different motility parameter (total, progressive, static and slow motility) varied significantly (p<0.01) irrespective of different freezing stages. Significantly higher progressive sperm motility and viability of buffalo spermatozoa were observed at fresh semen whereas lower progressive sperm motility and viability was found at post thaw stage. Total and progressive motility reduced by 2.5 and 2.12% following equilibration, whereas following cryopreservation, total and progressive motility reduced by 35.7 and 28.51% and static motility increases accordingly (35.4%). Significantly higher plasma membrane integrity of sperm was observed at fresh semen followed by pre freeze and post thaw semen. Following freezing, integrity of plasma membrane reduces at the rate of 10.81% and 26.7% at pre freezing and post thaw stages. Significantly higher average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), straightness (STR), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) were found for fresh semen followed by pre-freeze and post-thaw semen. Frozen buffalo semen with higher progressive motility and motion characteristics may be produced if motility losses can be reduced during freezing stage as this stage results higher motility losses. Asian Australas. J. Biosci. Biotechnol. 2022, 7(2), 75-81

Fresh and frozen semen quality of Murrah and Nili-Ravi bulls in comparison with indigenous buffalo bulls was evaluated by Computer Assisted Sperm Analyzer (CASA). Semen was collected regularly once a week semen collection schedule from six (two/group) buffalo bulls. Fresh semen was evaluated for sperm concentration, motility, sperm kinematics, morphology and dose/ejaculate. Semen was diluted in a commercial extender (AndroMed), equilibrated, cryopreserved and finally evaluated for post-thaw sperm quality. On-station Artificial Insemination (AI) was carried out in naturally estrus indigenous buffalo cows at their second or third parity. Semen volume and concentration did not vary significantly (p&gt;0.05). In fresh semen, total, static and slow motility differed significantly among the groups (p&lt;0.01). After cryopreservation, total, progressive and static motility shows significant differences (p&lt;0.01) among the groups followed by Indigenous, Murrah and Nili-Ravi. Indigenous buffalo bull has less morphological abnormalities than Murrah and Nili-Ravi bulls (p&lt;0.01). In fresh semen, VAP, VSL, STR, LIN and BCF differ significantly among the groups (p&lt;0.05) with higher values in indigenous bull followed by Nili-Ravi and Murrah. In frozen semen, STR, LIN, ALH and WOB differ significantly (p&lt;0.01) among the groups. Fertility did not vary significantly among the groups following 60 days post-AI. Irrespective of differences in fresh and frozen semen quality, all bulls from the three groups are suitable for AI with a considerable conception rate at least in on-station conditions. More dose/ejaculate can be produced from Murrah bulls (316.53±131.65) followed by Nili-Ravi (297.33±95.79) and Indigenous bulls (294.64±152.14). Murrah and Nili-Ravi breed can be used as similar efficiency of the indigenous bull for AI to increase the productivity of indigenous buffalo of Bangladesh.