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Aim: The aim of the present study is to access the effect of olive oil supplementation against high fat diet induced fatty liver disease in mice. Methods: Mice were divided into five groups: Group I (normal diet), Group II (high fat diet), Group III (olive oil), Group IV and V (High Fat Diet along with olive oil). All mice were fed for 16 weeks with weight measurements every 2 weeks and then sacrificed. Biochemical analysis of blood samples was done and mice livers were histologically examined. Results: Group II mice showed significant increase in body weight as compared with Group I (p < 0.05). Group IV and V mice were significantly (p < 0.05) reduced in body weight as compared with Group II. Olive oil groups had significantly decreased triglyceride and low density lipoprotein levels as compared with Group II whereas high density lipoprotein levels were significantly increased (p < 0.05). The liver enzymes were significantly increased in Group II as compared with other groups (p < 0.05). Liver histopathology revealed drastically increased lipid droplets in Group II mice as compared with Group IV & V. Conclusion: Olive oil causes weight reduction, decreases the serum triglycerides, normalizes the liver enzymes and significantly reduces the accumulation of fat in liver. Therefore, olive oil may represent a potential therapeutic alternative for NAFLD and other fatty diseases.

Green tea is a worldwide used beverage rich in fluoride and caffeine. To study the effect of excessive green tea intake versus fluoride and caffeine on body weight and serum thyroid hormones in male mice. Also, to confirm the effect of excessive green tea intake on thyroid gland was due to its fluoride and caffeine content or not. Thirty Male albino mice 25-30 gm weight were divided into five groups. Control (C) group (6 mice) receiving distilled water. Green tea (GT) treated group; 6 mice were given a 5 gm% tea hot water extract as their sole water supply. Fluoride (FL) treated group, 6 mice were given fluorine excess solution as their only water supply. Caffeine (Caf) treated group, 6 mice were treated with caffeine (6.25 mg/kg ip). Propylthiouracil (PTU) treated group, 6 mice were treated with PTU (10 mg/kg ip). Each of the five groups is treated with the corresponding substance for 30 days, then sacrificed under ether anesthesia, blood was collected to study serum levels of T3 and T4. There was a significant decrease in serum T3 in PTU treated group (0.72±0.03), GT treated group (0.96±0.04), FL treated group (0.93±0.04) and Caf treated group (1.46±0.08) in comparison to the C group (1.71±0.05).Also, there was a significant decrease in serum T4 in PTU treated group (15.71±1.26), GT treated group (30.07±2.29) and FL treated group (22.03±1.95)in comparison to the C group (51.1±3.15). But, there was an insignificant change in serum T4 in Caf treated group (45.55±2.73) in comparison to the C group (51.1±3.15). Green tea unfractionated aqueous extract decreased serum T3 and T4 and at the same time, increased final total body weight without a significant effect on the relative thyroid weight in comparison to the control group and this may be at least partly due to its content of fluoride and caffeine. Further work should be done on green tea free fluoride and caffeine to confirm this result.

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