This study demonstrates functional preservation and improved histological appearance of the injured glottis after a single treatment with topical mitomycin-C. Potential applications of these findings include prophylactic use of topical mitomycin-C on glottic insults that commonly progress from granulation tissue formation to scarring and decreased vocal fold function.

The development of multifunctional injectable adhesive hydrogels with self-healing capacity, shape adaptability, on-demand removability, and excellent photothermal antibacterial activity to promote bacteria-infected wound healing is highly recommended in practical applications. In this work, an injectable adhesive self-healing multiple-dynamic-bond crosslinked hydrogel was formed by a multiple-dynamic-bond crosslinked network of dynamic borate/didiol interactions, hydrogen bonding, and Schiff base bond. The introduction of Mussel-inspired catechol groups into the hydrogels could endow tissues with adhesive properties, and the hydrogel could adhere well to the skin under water with good shape adaptability under bent and twisted states. The mechanical and adhesive properties improved through the introduction of borate/didiol interactions into the catechol-modified hydrogel with dynamic Schiff base crosslinking at low cost and easy preparation, and the adhesive hydrogel could be removed without second damage to the wound. Moreover, polydopamine nanoparticles (PDA NPs) were introduced into the hydrogels through Schiff base reactions between the quinone group on PDA NPs and the primary amine in glycol chitosan (GC), resulting in an efficient photothermal antibacterial activity with uniformly dispersed PDA NPs in the hydrogel. And the hydrogels illustrated good cytocompatibility and hemocompatibility. Finally, they could be injected to fully fill irregular wounds and significantly promote bacteria-infected wound healing by reducing the inflammatory response, accelerating collagen deposition, and promoting blood vessel reconstruction. Therefore, this demonstrated their superiority in serving as multifunctional dressings for treating a bacteria-infected wound.

Injection of a hydrogel loaded with drugs with simultaneous anti-inflammatory and tissue regenerating properties can be an effective treatment for promoting periodontal regeneration in periodontitis. Nevertheless, the design and preparation of an injectable hydrogel with self-healing properties for tunable sustained drug release is still highly desired. In this work, polysaccharidebased hydrogels were formed by a dynamic cross-linked network of dynamic Schiff base bonds and dynamic coordination bonds. The hydrogels showed a quick gelation process, injectability, and excellent self-healing properties. In particular, the hydrogels formed by a double-dynamic network would undergo a gel−sol transition process without external stimuli. And the gel−sol transition time could be tuned by the double-dynamic network structure for in situ stimuli involving a change in its own molecular structure. Moreover, the drug delivery properties were also tunable owing to the gel−sol transition process. Sustained drug release characteristics, which were ascribed to a diffusion process, were observed during the first stage of drug release, and complete drug release owing to the gel−sol transition process was achieved. The sustained drug release time could be tuned according to the double-dynamic bonds in the hydrogel. The CCK-8 assay was used to evaluate the cytotoxicity, and the result showed no cytotoxicity, indicating that the injectable and self-healing hydrogels with double-dynamic bond tunable gel−sol transition could be safely used in controlled drug delivery for periodontal disease therapy. Finally, the promotion of periodontal regeneration in periodontitis in vivo was investigated using hydrogels loaded with ginsenoside Rg1 and amelogenin. Micro-CT and histological analyses indicated that the hydrogels were promising candidates for addressing the practical needs of a tunable drug delivery method for promoting periodontal regeneration in periodontitis.