In a large-scale study of socioeconomically homogeneous men that controlled for age, smoking, and other cardiovascular risk factors, we found no evidence of association between Chlamydia pneumoniae IgG seropositivity and risks of future MI.

Ureaplasma urealyticum infection is associated with premature onset of labor and with increasing duration of time between rupture of membranes and delivery. Eradication of ureaplasmas from the urogenital tract of women and their partners, ideally before conception, should be considered.

The antimicrobial activity of rosoxacin, a new quinoline antibacterial compound, was determined against the causative organisms of three sexually transmitted diseases. Rosoxacin demonstrated a high degree of activity against Neisseria gonorrhoeae clinical isolates, with the miniimal inhibitory concentrations for 50% of these being 0.03 ,ug/ml. The corresponding miniimal inhibitory concentrations for penicillin, ampicillin, tetracycline, and spectinomycin were 0.25 U/ ml, 0.125 ug/ml, 0.25 ,tg/ml, and 16 ,ug/ml, respectively. Eleven strains of Chlamydia trachomatis were inhibited by 5 ,ug of rosoxacin per ml, and each of seven Ureaplasma urealyticum strains was inhibited by 2 to 8 Ag of rosoxacin per ml. The results of these susceptibility studies, coupled with those of an earlier evaluation of the phannacokinetics of rosoxacin, provide support for extending or undertaking clinical evaluations of this compound against infections with N. gonorrhoeae, C. trachomatis, and U. urealyticum.Rosoxacin [1-ethyl-1,4-dihydro-4-oxo-7-(4-pyridinyl)-3-quinoline-carboxylic acid] (Fig. 1 Susceptibility tests were performed by an agar dilution method employing the foilowing medium: GC agar base (BBL) containing 1% hemoglobin (BBL) and 1% IsoVitaleX (BBL). Twofold serial concentrations of the antibacterial agents were incorporated into complete GC agar medium and poured into plastic petri dishes (100 by 15 mm; Fisher Scientific Co.). The cultures were grown on the surface of complete GC agar medium plates for 24 h at 37°C in a candle jar. Growth on the surface of each plate was washed off with Trypticase soy broth. Each suspeIsion was shaken with glass beads for 5 min on a paint shaker. The suspensions were adjusted spectrophotometrically with Trypticase soy broth to contain 10" colonyforming units per ml. The adjusted suspensions were further diluted in broth to contain 4 x 106 colonyforming units per ml. Samples (0.5 ml each) of the diluted cultures were added to the wells of a Steers replicator (8). Each point of the replicator delivered 0.0025 ml (104 colony-forming units per spot). All plates were inoculated with the replicator and incubated for 24 h at 370C in candle jars. The minimal inhibitory concentration (MIC) was read as the lowest concentration of antibacterial drug that completely inhibited growth.

Controversy over the association of Ureaplasma urealyticum with reproductive failure may be due to methods used to isolate the microorganism. U. urealyticum isolations from clinical material should be done simultaneously in broth and on Shepard's differential agar medium (A7) containing manganese sulfate. Urine sediments result in a 9% (P = 0.0002) higher rate of isolation than than cervical and urethral swabs. Primary isolations may not display standard textbook morphology. Isolated colonies may be present, but brown streaks in cervical mucus or a coalescent haze around epithelial cells in urine sediment may also be seen in areas of concentrated growth. The broth and agar media used, method of incubation, type of specimen, and method of storing specimens before culture are all factors which influence the recovery of U. urealyticum.