Black raspberries (Rubus occidentalis L.) are rich in phytonutrients that have demonstrated chemoprotective properties against certain degenerative diseases. To estimate variability in phytonutritional quality among sources of black raspberry, 19 samples representing four common midwestern cultivars obtained from eight production sites were assayed for their antioxidant capacity [2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing ability of plasma (FRAP) methods], total phenolic content (TP), total monomeric anthocyanin levels (TMA), and levels of cyanidin 3-rutinoside. The antioxidant potential among samples averaged 2.92 ± 0.29 and 4.62 ± 0.88 mmol TE·100 g−1 fresh weight by the DPPH and FRAP methods, respectively; TP, TMA, and cyanidin 3-rutinoside means averaged 449 ± 62, 336 ± 109, and 244 ± 84 mg·100 g−1 fresh weight, respectively. Levels of FRAP, TP, TMA, and cyanidin 3-rutinoside were strongly correlated (r = +0.85 to +0.96). Mean antioxidant capacities and phenolic constituent levels were similar among ‘Bristol’, ‘Jewel’, and ‘MacBlack’ samples; values for a single sample of ‘Haut’ were lower but comparable to levels found in individual samples of the other three cultivars. Black raspberry production site differences were statistically significant for FRAP, TMA, cyanidin 3-rutinoside, and titratable acidity (TA) levels. Inverse relationships (r = –0.65 to –0.74) among black raspberry samples for FRAP, TMA, or cyanidin 3-rutinoside levels versus levels of TA suggested that site differences may be partially attributable to fruit ripeness at harvest. Relationships among these parameters versus regional differences in soil temperatures were also significant but weak. Regardless of its environmental or physiological drivers, point-source variation in fruit phytonutrient contents may be a relevant concern in health-related studies or clinical applications. Moreover, it may impact the nutritional benefits to the consumer and affect the quality advantages associated with direct-marketed black raspberries.

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Root development and the associated acquisition of water and nutrients are an important part of weed competitiveness. Characterization of root morphological development, however, is inherently problematic because of the complexities of soil–plant interactions. In this study, we used hydroponically grown plants and digital imaging to examine root characteristics ofGlycine maxand the competing weedsSenna obtusifoliaandAmaranthus palmeri. The purpose was to define inherent differences in root length and surface area that would contribute to growth responses during the establishment phase in the field. The methodology involved growing plants for 16 to 22 d, dissecting and staining root segments, mounting subsamples on slides, and imaging using a stereomicroscope and digital camera. Microscopy was required because of the small diameters of a significant proportion of the weed roots. With plants of similar root fresh weights (4.5 to 5.0 g), counting of individual roots revealed that 5.obtusifoliaandA. palmerihad 2 and 3.7 times more roots thanG. max(4,616 and 7,781 vs. 2,120, respectively). The imaging analyses indicated that roots ofS. obtusifoliaandA. palmerihad 2.9 and 5 times more length thanG. max(10,042 and 17,192 cm vs. 3,418 cm, respectively). Furthermore, the analysis of length in different root diameter classes indicated that weed roots were noticeably finer then those ofG. max. Approximately 84% ofS. obtusifoliaroot length was contributed by roots in the 0.1- to 0.25-mm range, whereas 45% of theG. maxroots were in the 0.1- to 0.25-mm range and 48% were in the 0.25- to 0.75-mm range. In contrast, 68% ofA. palmerilength was contributed by roots smaller than 0.1 mm in diameter with 26% in the 0.1- to 0.25-mm range. Based on the expression of root characteristics observed here, root systems of these weed species would have finer roots with much greater length that would occupy a much larger volume of soil than those ofG. max. Presumably, this would result in a competitive advantage in the acquisition of water and nutrients, especially when availability is limited.

Experiments were conducted to compare germination efficiencies and vegetative growth of soybean and the competing weed species, sicklepod and Palmer amaranth, over a range of temperatures in the root zone and aerial environments. From genetic origins we hypothesized that the weeds would have a higher temperature optimum, which would help explain competitive interactions seen in the southeastern U.S. Germination experiments indicated that germination efficiency of the weeds was much more sensitive to low temperature than soybean, being markedly inhibited below 18 C. Similarly, experiments in an automated, temperature-controlled hydroponic system revealed that the weed species were less tolerant of low root zone temperature but more tolerant of high root zone temperature than soybean. At 16 C, dry weight of soybean was 74% of the control dry weight at 24 C, whereas dry weights of sicklepod and Palmer amaranth were 5 and 20% of the control, respectively. At 32 C, soybean root dry weight was only 80% of the 24 C treatment, whereas root dry weight of the weed species was not significantly different. When plants were grown at a low aerial temperature, growth of all plants was strongly inhibited] but the negative effects were somewhat more severe in the weed species than with soybean. An increase in aerial temperature from 26/22 C to 34/30 C (day/night) had a positive influence on dry matter accumulation of the weed species, stimulating sicklepod 150 to 200% and Palmer amaranth 150 to 1,600% compared to their respective controls, whereas soybean remained at about 80 to 90% of the control. All species grew taller with increasing temperature. Leaf area of the weeds increased but leaf area of soybean did not increase. Consistent with our original hypothesis, the results clearly show that the weeds, which originate from warm geographical regions, respond more negatively than soybean to low temperatures in the growth environment but more positively to high temperatures. The temperature characteristics help to explain why the intensity of weed pressure increases as the soybean growing season progresses, even after canopy closure.

No abstract