Rhamnolipid (RL) biosurfactant which is produced by Pseudomonas species is one of the most effective surface-active agents investigated in the literature.

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The aim of this study was to develop a sensitive lateral flow immunoassay (LFIA) for the rapid detection of Escherichia coli (E. coli) O157:H7, a pathogen contributor to diseases and fatalities worldwide. Au nanoparticles with high stability, uniform size, and shape were synthesized and coated with heterobifunctional PEG polymer with carboxyl groups, and they were bioconjugated to be used as label in sandwich-LFIA. Then, a silver enhancement strategy was developed as an accessible, rapid, and cost-effective approach for signal amplification to reduce the limit of detection (LOD). The optimal results were achieved when a solution of silver nitrate and hydroquinone/citrate buffer was added to the strips for 4 min. This led to a decrease in the visual LOD from 2 × 106 (CFU mL−1) to 2 × 103 (CFU mL−1), resulting in a threefold improvement in sensitivity compared to the conventional LFIA system. The specificity of the system was evaluated by using non-target bacteria (E. coli BL21 and E. coli T515) and its reliability was determined by testing commercial food samples (milk, tap water, and orange juice), demonstrating its effectiveness for quickly detecting pathogenic bacteria in food products. Graphical Abstract

An efficient lipopeptide biosurfactant (BS) producer, Aneurinibacillus thermoaerophilus HAK01, was isolated from municipal landfill sites.

Biofilms find a favorable environment in industrial processes such as food, cosmetic, or medical prosthesis and devices, being responsible of approximately 80% of human bacterial infections. Prevention and/or eradication of microorganism’ films is a worldwide need. There is an increasing interest on the finding and use of novel antimicrobial compounds without side effects. An additional challenge is to fight the antimicrobial resistance that some bacteria and microorganisms develop with traditional antibiotics. Also, in recent years, sustainability and natural source of the antibiofilm chemical principles are also a priority demand. Colloidal systems such as vesicles, particle suspensions, or emulsions are becoming increasingly useful tools for biocompound delivery due to their ability to protect the compound encapsulated against external factors and their possibility to be used as target delivery systems. During the last decade, these types of systems have been widely used for the encapsulation of traditional and novel compounds with antimicrobial properties. The present study summarizes different types of natural compounds tested against several types of bacteria and their feasibility to be encapsulated in different types of colloidal systems.