Overproduction of rhamnolipid by fed-batch cultivation of Pseudomonas aeruginosa in a lab-scale fermenter under tight DO control

Bazsefidpar, Shayesteh; Mokhtarani, Babak; Panahi, Reza; Hajfarajollah, Hamidreza

doi:10.1007/s10532-018-09866-3

Cited by 25 publications

(13 citation statements)

References 31 publications

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“…Thus, extension of production cycle and maintenance of high productivity at late stage are important to improve the rhamnolipids production efficiency. Considering these, fed-batch fermentation was considered as more efficient in maintaining the biomass growth rate and rhamnolipids productivity [19,[21][22][23]. However, rhamnolipids are both the target product and major contributor to severe foam problems, and more rhamnolipids production would cause more severe foam problem, needing more efficient foam-controlling strategy [6].…”

Section: Introductionmentioning

confidence: 99%

Enhanced rhamnolipids production using a novel bioreactor system based on integrated foam-control and repeated fed-batch fermentation strategy

Shi-xun

et al. 2020

Biotechnol Biofuels

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Background: Rhamnolipids are the best known microbial-derived biosurfactants, which has attracted great interest as potential ''green" alternative for synthetic surfactants. However, rhamnolipids are the major contributors to severe foam problems, which greatly inhibit the economics of industrial-scale production. In this study, a novel foam-control system was established for ex situ dealing with the massive overflowing foam. Based on the designed facility, foam reduction efficiency, rhamnolipids production by batch and repeated fed-batch fermentation were comprehensively investigated. Results: An ex situ foam-control system was developed to control the massive overflowing foam and improve rhamnolipids production. It was found that the size of individual bubble in the early stage was much larger than that of late fermentation stage. The foam liquefaction efficiency decreased from 54.37% at the beginning to only 9.23% at the end of the fermentation. This difference of bubble stability directly resulted in higher foam reduction efficiency of 67.46% in the early stage, whereas the small uniform bubbles can only be reduced by 57.53% at the later fermentation stage. Moreover, reduction of secondary foam is very important for foam controlling. Two improved designs of the device in this study obtained about 20% improvement of foam reduction efficiency, respectively. The batch fermentation result showed that the average volume of the overflowing foam was reduced from 58-640 to 19-216 mL/ min during the fermentation process, presenting a notable reduction efficiency ranging from 51.92 to 73.47%. Meanwhile, rhamnolipids production of batch fermentation reached 45.63 g/L, and the yield 0.76 g/g was significantly better than ever reported. Further, a repeated fed-batch fermentation based on the overall optimization was carried out. Total rhamnolipids concentration reached 48.67 g/L with the yield around of 0.67-0.83 g/g, which presented an improvement of 62% and 49% compared with conventional batch fermentation by using various kinds of defoamers, respectively. Conclusions: The ex situ foam-control system presented a notable reduction efficiency, which helped greatly to easily solve the severe foaming problem without any defoamer addition. Moreover, rhamnolipids production and yield

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Section: Introductionmentioning

confidence: 99%

Enhanced rhamnolipids production using a novel bioreactor system based on integrated foam-control and repeated fed-batch fermentation strategy

Shi-xun

et al. 2020

Biotechnol Biofuels

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“…Biosurfactants (BS) are known as natural surfactants produced by bacteria, fungi, and yeast. 1 BS has many advantages compared to chemically synthesized surfactants such as high biodegradability, low toxicity, production from renewable sources and stable activity under harsh environmental conditions. They can be used in the food, agricultural, pharmaceutical, oil and petrochemical industries.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical characterization and optimization of glycolipid biosurfactant production by a native strain ofPseudomonas aeruginosaHAK01 and its performance evaluation for the MEOR process

et al. 2019

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“…Importantly, only three parameters were considered in our study for the optimization of culture conditions (carbon source, nitrogen source and production time). Thus, additional improvements could be made in the rhamnolipid yield obtained using waste canola oil as a carbon source through the optimization of other important bioprocess parameters, such as pH, incubation temperature and dissolved oxygen among others (Zhu et al 2012 ; Müller et al 2012 ; Bazsefidpar et al 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Use of waste canola oil as a low-cost substrate for rhamnolipid production using Pseudomonas aeruginosa

et al. 2019

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Rhamnolipids are glycolipid biosurfactants that are primarily produced by Pseudomonas aeruginosa that have gained a great deal of interest for their numerous industrial applications and environmentally friendly properties. In this study, we explored the potential of waste canola oil as a low-cost and environmentally friendly substrate for the production of rhamnolipids by P. aeruginosa . Four different 2 3 full factorial designs were used to assess the effect of three independent factors on rhamnolipid production, including carbon source (canola oil and waste canola oil), nitrogen source [(NH 4 ) 2 SO 4 and NaNO 3 ] and production time (7 and 14 days). The highest observed yield was 3585.31 ± 66.24 mg/L when P. aeruginosa was cultured for 14 days with 3% v/v waste canola oil and 4 g/L of NaNO 3 . The nitrogen source proved to be a crucial factor, as the use of NaNO 3 rather than (NH 4 ) 2 SO 4 led to a 30-fold increase in production yield. The observed yield when waste canola oil was used was similar to, and even slightly higher than, that obtained using canola oil. Our results showed that waste canola oil has great potential for use as a carbon source for rhamnolipid production by P. aeruginosa , thus paving the way for the development of a low-cost, efficient, and environmentally friendly bioprocess for the production of rhamnolipids. Electronic supplementary material The online version of this article (10.1186/s13568-019-0784-7) contains supplementary material, which is available to authorized users.

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Overproduction of rhamnolipid by fed-batch cultivation of Pseudomonas aeruginosa in a lab-scale fermenter under tight DO control

Cited by 25 publications

References 31 publications

Enhanced rhamnolipids production using a novel bioreactor system based on integrated foam-control and repeated fed-batch fermentation strategy

Enhanced rhamnolipids production using a novel bioreactor system based on integrated foam-control and repeated fed-batch fermentation strategy

Physicochemical characterization and optimization of glycolipid biosurfactant production by a native strain ofPseudomonas aeruginosaHAK01 and its performance evaluation for the MEOR process

Use of waste canola oil as a low-cost substrate for rhamnolipid production using Pseudomonas aeruginosa

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