In the present study the crude oil and defatted seed cake were analyzed to calculate phenolics, flavonoid, totaltocopherol and DPPH activity for two locations. The C. fistula had highest phenolics 12.0±0.0 mgGAE/g, flavonoids content 2.9±0.0 mgCAE/g, total tocopherol 185.6 mg/g and carotenoids was 131.0 mg/kg. The maximum antioxidant activity was 83% and in terms of IC 50 value was 0.041±0.0 mg/ml. The fatty acids profile shown that the significant amount of fatty acids were linoleic and oleic acid.