BackgroundWhartons jelly-derived mesenchymal stem cells are a valuable alternative source that possess multipotent properties, easy to obtain and available in large scale compared to BMMSCs. We investigated the possibility of cardiac function improvement post isoproterenol induced cardiac injury in a rat model following human WJMSCs transplantation.Materials and MethodsMSCs were extracted and cultured from cord WJ, characterized by morphology, Immunophenotyping and differentiation to osteoblast and adipocytes. WJMSCs were labeled with PKH2 linker dye. Wistar rats were divided into control group, ISO group (injected with 2 doses of isoproterenol) to induce myocardial injury and ISO group transplanted with labelled WJMSCs. ECG, electrocardiographic patterns, cardiac marker enzymes, tracing of labeled MSCs and immunohistochemical analysis of myocardial cryosections were studied.Results and ConclusionsWJ derived MSCs were expanded for more than 14 passages while maintaining their undifferentiated state, were positive for MSC markers and were able to differentiate into adipocyte and osteoblast. We demonstrated that intravenously administered WJMSCs were capable of homing predominently in the ischemic myocardium. Cardiac markers were positively altered in stem cell treated group compared to ISO group. ECG and ECHO changes were improved with higher survival rate. WJMSCs could differentiate into cardiac-like cells (positive for cardiac specific proteins) in vivo. WJMSCs infusion promoted cardiac protection and reduced mortality, emphasizing a promising therapeutic role for myocardial insufficiency.

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Background: Many oral airways are used for aiding fiberoptic bronchoscope (FOB)-guided endotracheal intubation. This study was done to evaluate modified William's airway, modified Guedel's airway, and LMA MADgic airway as conduits for FOB-guided endotracheal intubation. Methods: Sixty patients presented for elective surgery under general anesthesia were randomly allocated into three groups: Modified Guedel's airway group (G G), modified Williams airway group (G W), and LMA MADgic airway group (G M). The three study groups were compared with regard to time of insertion of the airway, time of intubation, ease of airway insertion, number of intubation attempts, Laryngeal View Grade (LVG), and the incidence of complications. Results: G w had shorter time of airway insertion, shorter time of intubation, lower number of intubation attempts, and better laryngeal view compared to G G and G M. The anesthesiologist was more comfortable in G w compared to the other two groups.The incidence of complications (sore throat, and bloodstained airway) was comparable between the three groups. Conclusion: Modified Williams airway provided shorter time of endotracheal tube intubation, and lower number of intubation attempts in comparison to modified Guedel's airway and LMA MADgic airway when used as conduit for FOB-guided endotracheal intubation. This randomized controlled study was conducted in Cairo University Hospital. Research Ethics Committee approved the study (N-40-2016).