Four novel triterpene glycosides named bodinitins A (1), B (2), C (3), and D (4) were isolated from the roots of Schefflera bodinieri. Based on spectroscopic data, especially 1H−H COSY and 13C−1H COSY NMR, the structures of the glycosides have been determined as 28-O-[α-l-rhamopyranosyl-(1→4)-O-β-d-glucopyranosyl-(1→6)]-β-d-glucopyranoside of demethylisoaleuritolic acid (1), 28-O-[α-l-rhamopyranosyl(1→4)-O-β-d-glucopyranosyl(1→6)]-β-d-glucopyranoside of isoaleuritolic acid (2), 28-O-[α-l-rhamnopyranosyl(1→4)-O-β-d-glucopyranosyl(1→6)]-β-d-glucopyranoside of 3-oxo-8-demethylisoaleuritolic acid (3), and 28-O-[α-l-rhamopyranosyl(1→4)-O-β-d-glucopyranosyl(1→6)]-β-d-glucopyranoside of 3-oxoisoaleuritolic acid (4).

A sequence characterised amplified region marker was developed to determine an acid resistance-related gene in Lactobacillus plantarum. A random amplified polymorphic DNA marker named S116-680 was reported to be closely related to the acid resistance of the strains. The DNA band corresponding to this marker was cloned and sequenced with the induction of specific designed PCR primers. The results of PCR test helped to amplify a clear specific band of 680 bp in the tested acid-resistant strains. S116-680 marker would be useful to explore the acid-resistant mechanism of L. plantarum and to screen desirable malolactic fermentation strains.

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