We evaluated whether insulin could stimulate β-alanine uptake by skeletal muscle cells in vitro. Mouse myoblasts (C2C12) (n = 3 wells per condition) were cultured with β-alanine (350 or 700 µmol·L−1), with insulin (100 µU·mL−1) either added to the media or not. Insulin stimulated the β-alanine uptake at the lower (350 µmol·L−1) but not higher (700 µmol·L−1) β-alanine concentration in culture medium, indicating that transporter saturation might blunt the stimulatory effects of insulin.