Background: Urinary tract infection is the most common community and hospital bacterial infection and characterized by high rate of treatment failure and recurrences. Aim: to determine the association between risk factors and bacterial type. Materials and Methods: A prospective cross-sectional study conducted during the period from 1st of June 2015 to the end of January 2016. The population included in the study are 563 women, of them 425 (75.5%) were outpatients, and 138 (24.5%) were inpatients. Their age range between 18 and 80 years, with a mean age of 33.59±15.29 years. Urine samples were immediately cultured on blood agar and MacConkey's agar by spread plate technique. Bacterial colonies with different morphology were selected, purified and identified according to their biochemical characteristics using conventional standard methods. Results: Mean age was significantly (F=5.14, P=0.002) different in relation to bacterial type. Women infected with E. coli were with higher mean age (37.84 year), followed by those infected with Staphylococcus aureus (31.97 year), then Klebsiella pneumonia (28.76 year) and Proteus mirabilis (28.50 year). BMI mean value was significantly (F=6.33, P=0.000) different in women infected with different bacteria and higher value was in those infected with E. coli (26.15), while it was about the same in those infected with Staphylococcus aureus (24.6), then Klebsiella pneumonia (24.9 year) and Proteus mirabilis (24.1). Pus cell scale mean value was significantly (F=6.67, p=0.000) higher in cases infected with E. coli (2.04), while 1.77 in Staphylococcus aureus, infected cases, 1.15 in women infected with Klebsiella pneumonia and 1.33 in those infected with Proteus mirabilis. Conclusion: Age, BMI, pus cells scale, and education levels were significantly associated with bacterial type.
Background: Urinary tract infection is common with health impact in women and characterised by failure to treatment and recurrent episodes. Aim: This study was conducted to determine the risk factors for the development of urinary tract infection in diabetic and pregnant women in comparison to student female. Materials and methods: A prospective cross-sectional study conducted during the period from 1st of June 2015 to the end of January 2016. The population included in the study are 563 women, of them 425 were outpatients, and 138 were inpatients. Their age range between 18 and 80 years, with a mean age of 33.59±15.29 years. Urine samples collected and cultured on blood agar and MacConkey agar by spread plate technique. Bacterial colonies with different morphology were selected, purified and identified according to their biochemical characteristics using conventional standard methods. Results: In diabetic women, there were no significant difference in mean age and BMI values between culture positive and culture negative groups. However, pus cell mean scale was significantly higher [P=0.000] in women with urinary tract infection [1.76±1.25] than in those with negative culture [0.69±1.00]. In pregnant women, BMI mean value was significantly [P=0.013] lower in pregnant women with UTI [26.14] as compared to those without infection [26.99]. Pus cell scale mean value was significantly [P=0.000] higher in pregnant women with UTI [1.55] than women with negative UTI [0.85]. While there was no significant difference in mean age between UTI positive and negative pregnant women. In female student, there was a significant difference between UTI infected and non-infected in mean age [P=0.041] and pus cell scale [P=0.000]. However, BMI was not significantly different between infected and non-infected female student. Other risk factors association are variables in the 3 groups when analysed using X2, while AUC and OR show different trends of association between risk factors and UTI. Conclusion: BMI, pus cell scale, child number, delivery method, operation history and hospital setting were significantly associated with culture positivity in the 3 studied groups as determined by AUC. While OR confirmed association with pus sale scale in the 3 groups.
Background: Urinary tract infections form the common community and hospital acquired- infection in Iraq, with impact on health care delivery. The emergence of antimicrobial resistance which increased in its prevalence with time attributed the treatment failure and recurrent infection. Aim: The aim of this study was determine the prevalence of extended spectrum beta lactamase and ESBL resistance genes of E. coli in female in Kirkuk city.
Background: Antibiotic resistance emerged as clinical problem challenge the effective treatment of infections. Virulence factor may play an important role in the influence of antimicrobial resistance. Objective: To determine the frequency of resistance gene in E. coli clinical isolates from women with urinary tract infection. Materials and Methods: Fifteen E.coli clinical isolates were tested by PCR to determine their molecular characterization. Results: The bla CTX –M gene was not detected in 6.7% out of the tested 15 E. coli clinical isolates from women with urinary tract infection. However, bla OXA gene was detected in all E. coli tested clinical isolates from pregnant women, female student and diabetic women with urinary tract infection. While bla TEM gene and bla SHV gene were not detected in 33.3% and 40% out of the tested E. coli clinical isolates respectively. Conclusions: Four types of ESBL genes were detected, and shows new trend of distribution, which indicated the predominance of OXA and CTX-M genes.
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